TY - JOUR
T1 - OECD validation study to assess intra- and inter-laboratory reproducibility of the zebrafish embryo toxicity test for acute aquatic toxicity testing
AU - Busquet, François
AU - Strecker, Ruben
AU - Rawlings, Jane M.
AU - Belanger, Scott E.
AU - Braunbeck, Thomas
AU - Carr, Gregory J.
AU - Cenijn, Peter
AU - Fochtman, Przemyslaw
AU - Gourmelon, Anne
AU - Hübler, Nicole
AU - Kleensang, André
AU - Knöbel, Melanie
AU - Kussatz, Carola
AU - Legler, Juliette
AU - Lillicrap, Adam
AU - Martínez-Jerónimo, Fernando
AU - Polleichtner, Christian
AU - Rzodeczko, Helena
AU - Salinas, Edward
AU - Schneider, Katharina E.
AU - Scholz, Stefan
AU - van den Brandhof, Evert Jan
AU - van der Ven, Leo T.M.
AU - Walter-Rohde, Susanne
AU - Weigt, Stefan
AU - Witters, Hilda
AU - Halder, Marlies
N1 - Funding Information:
Melanie Knöbel was financially supported as part of the CEFIC and U.K. DEFRA funded CEllSens-project ( CEFIC-LRI Eco8/8.2 ), co-ordinated by Kristin Schirmer (Eawag). Evert-Jan van den Brandhof and Leo T.M. van der Ven were financially supported by the Ministry of Infrastructure and the Environment (NL) , project REACH ( 601351 ). Fernando Martínez-Jerónimo was financially supported by the Instituto Politécnico Nacional-E.N.C.B . Juliette Legler was supported by a grant from the Netherlands Organization for Scientific Research (Grant No. 864.09.005 ).
PY - 2014/8
Y1 - 2014/8
N2 - A The OECD validation study of the zebrafish embryo acute toxicity test (ZFET) for acute aquatic toxicity testing evaluated the ZFET reproducibility by testing 20 chemicals at 5 different concentrations in 3 independent runs in at least 3 laboratories. Stock solutions and test concentrations were analytically confirmed for 11 chemicals. Newly fertilised zebrafish eggs (20/concentration and control) were exposed for 96. h to chemicals. Four apical endpoints were recorded daily as indicators of acute lethality: coagulation of the embryo, lack of somite formation, non-detachment of the tail bud from the yolk sac and lack of heartbeat. Results (LC50 values for 48/96. h exposure) show that the ZFET is a robust method with a good intra- and inter-laboratory reproducibility (CV. <. 30%) for most chemicals and laboratories. The reproducibility was lower (CV > 30%) for some very toxic or volatile chemicals, and chemicals tested close to their limit of solubility. The ZFET is now available as OECD Test Guideline 236.Considering the high predictive capacity of the ZFET demonstrated by Belanger et al. (2013) in their retrospective analysis of acute fish toxicity and fish embryo acute toxicity data, the ZFET is ready to be considered for acute fish toxicity for regulatory purposes.
AB - A The OECD validation study of the zebrafish embryo acute toxicity test (ZFET) for acute aquatic toxicity testing evaluated the ZFET reproducibility by testing 20 chemicals at 5 different concentrations in 3 independent runs in at least 3 laboratories. Stock solutions and test concentrations were analytically confirmed for 11 chemicals. Newly fertilised zebrafish eggs (20/concentration and control) were exposed for 96. h to chemicals. Four apical endpoints were recorded daily as indicators of acute lethality: coagulation of the embryo, lack of somite formation, non-detachment of the tail bud from the yolk sac and lack of heartbeat. Results (LC50 values for 48/96. h exposure) show that the ZFET is a robust method with a good intra- and inter-laboratory reproducibility (CV. <. 30%) for most chemicals and laboratories. The reproducibility was lower (CV > 30%) for some very toxic or volatile chemicals, and chemicals tested close to their limit of solubility. The ZFET is now available as OECD Test Guideline 236.Considering the high predictive capacity of the ZFET demonstrated by Belanger et al. (2013) in their retrospective analysis of acute fish toxicity and fish embryo acute toxicity data, the ZFET is ready to be considered for acute fish toxicity for regulatory purposes.
KW - Alternative method
KW - Aquatic acute toxicity
KW - Reproducibility
KW - Validation
KW - Zebrafish embryo
UR - http://www.scopus.com/inward/record.url?scp=84904057656&partnerID=8YFLogxK
U2 - 10.1016/j.yrtph.2014.05.018
DO - 10.1016/j.yrtph.2014.05.018
M3 - Artículo
SN - 0273-2300
VL - 69
SP - 496
EP - 511
JO - Regulatory Toxicology and Pharmacology
JF - Regulatory Toxicology and Pharmacology
IS - 3
ER -