TY - JOUR
T1 - Occurrence and infective potential of Colletotrichum gloeosporioides isolates associated to Citrus limon var Eureka
AU - Patricia, Larralde Corona Claudia
AU - María del Socorro, Ramírez González
AU - Iván, Rosas Hernández
AU - Erika, De la Cruz Arguijo
AU - Carolina, De Santiago
AU - Keiko, Shirai
AU - José Alberto, Narváez Zapata
N1 - Publisher Copyright:
© 2021
PY - 2021/9
Y1 - 2021/9
N2 - A collection of 37 fungi associated to Italian lemon plants with disease symptoms, was obtained. Ten genera including Aspergillus, Alternaria, Nigrospora, Lasiodiplodia, Dothideomycetes, Pleurostoma, Setosphaeria, Penicillium, Fusarium and Colletotrichum were identified by using ITS1–5.8S–ITS2, D1/D2 26S and COX1 loci. The last three genera were abundant on the damaged fruits, being Colletotrichum the more abundant (32.4 %). CaInt2 and CgInt primers support the identity of these isolates as C. gloeosporioides. Variability, inferred by rep-PCR and multilocus sequence analysis shows genetic differences among the C. gloeosporioides isolates. Infective profile evaluated in Colletotrichum isolates shows different leave infection percentages (26 to 60 %). SEM analysis showed mycelium, spores and appressoria on the leaves of selected Colletotrichum isolates. Specifically, the AL-05 and AL-13 isolates showed a high chitin deacetylase activity (CDA) peaking at 1.2 U/mg protein in AL-13. This is the first report on C. gloeosporioides infecting Italian lemon leaves in Mexico.
AB - A collection of 37 fungi associated to Italian lemon plants with disease symptoms, was obtained. Ten genera including Aspergillus, Alternaria, Nigrospora, Lasiodiplodia, Dothideomycetes, Pleurostoma, Setosphaeria, Penicillium, Fusarium and Colletotrichum were identified by using ITS1–5.8S–ITS2, D1/D2 26S and COX1 loci. The last three genera were abundant on the damaged fruits, being Colletotrichum the more abundant (32.4 %). CaInt2 and CgInt primers support the identity of these isolates as C. gloeosporioides. Variability, inferred by rep-PCR and multilocus sequence analysis shows genetic differences among the C. gloeosporioides isolates. Infective profile evaluated in Colletotrichum isolates shows different leave infection percentages (26 to 60 %). SEM analysis showed mycelium, spores and appressoria on the leaves of selected Colletotrichum isolates. Specifically, the AL-05 and AL-13 isolates showed a high chitin deacetylase activity (CDA) peaking at 1.2 U/mg protein in AL-13. This is the first report on C. gloeosporioides infecting Italian lemon leaves in Mexico.
KW - CDA activity
KW - Colletotrichum
KW - Italian lemon
KW - Pathogenic potential
UR - http://www.scopus.com/inward/record.url?scp=85111058846&partnerID=8YFLogxK
U2 - 10.1016/j.btre.2021.e00651
DO - 10.1016/j.btre.2021.e00651
M3 - Artículo
AN - SCOPUS:85111058846
SN - 2215-017X
VL - 31
JO - Biotechnology Reports
JF - Biotechnology Reports
M1 - e00651
ER -