Obtaining mutants for protoplast fusion of gibberellin-forming Gibberella fujikuroi strains.

K. B. Torres, B. Brückner, B. Meier

Research output: Contribution to journalArticlepeer-review

Abstract

Auxotrophic, drug-resistant, nitrate-nonutilizing, and albino mutants have been isolated in Gibberella fujikuroi following UV mutagenesis. Protoplasts of complementing auxotrophic strains, mutants with resistance markers, or mutants blocked in different steps of the nitrate assimilatory pathway have been fused to form heterokaryons, diploids, or recombinant haploids. The properties of fusant strains, including gibberellic acid productivity, have been examined and compared to parent strains.
Original languageAmerican English
Pages (from-to)83-95
Number of pages13
JournalApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
StatePublished - 1 May 1992
Externally publishedYes

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