TY - JOUR
T1 - New microsatellite loci for estimating genetic diversity and structure in Octopus hubbsorum from Nayarit, México
AU - de Jesús Dueñas-Romero, José
AU - Domínguez-Contreras, José Francisco
AU - Granados-Amores, Jasmín
AU - Munguía-Vega, Adrián
AU - García-Rodríguez, Francisco Javier
N1 - Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer Nature B.V.
PY - 2021/10
Y1 - 2021/10
N2 - Background: Octopus hubbsorum Berry, 1953 is the most important species for commercial fishing in the Mexican Pacific. However, there is a lack of information regarding population structure that could have important management implications. We tested 44 microsatellite loci in O. hubbsorum by cross-amplification from O. bimaculatus. Methods and results: Genetic diversity and structure was tested over 30 octopus sampled from Santa Cruz de Miramar (Nayarit, México). A total of 11 loci were successfully amplified. All loci were polymorphic with the number of effective alleles ranging from 2.13 to 23.14, while three loci significantly deviated from Hardy-Weinberg equilibrium. No significant LD was observed between pairs of loci (P ≥ 0.05). The application of the new markers in a O. hubbsorum population from Santa Cruz de Miramar Nayarit, México, did not showed Wahlund or isolate breaking effects due to the mixing of distinct populations. Conclusions: The loci were useful to estimate levels of pairwise relatedness and to discard the presence of recent demographic bottlenecks in the population.
AB - Background: Octopus hubbsorum Berry, 1953 is the most important species for commercial fishing in the Mexican Pacific. However, there is a lack of information regarding population structure that could have important management implications. We tested 44 microsatellite loci in O. hubbsorum by cross-amplification from O. bimaculatus. Methods and results: Genetic diversity and structure was tested over 30 octopus sampled from Santa Cruz de Miramar (Nayarit, México). A total of 11 loci were successfully amplified. All loci were polymorphic with the number of effective alleles ranging from 2.13 to 23.14, while three loci significantly deviated from Hardy-Weinberg equilibrium. No significant LD was observed between pairs of loci (P ≥ 0.05). The application of the new markers in a O. hubbsorum population from Santa Cruz de Miramar Nayarit, México, did not showed Wahlund or isolate breaking effects due to the mixing of distinct populations. Conclusions: The loci were useful to estimate levels of pairwise relatedness and to discard the presence of recent demographic bottlenecks in the population.
KW - Genetic diversity
KW - Isolate Breaking Effect
KW - Mexican Pacific
KW - Octopus
KW - Wahlund Effect
UR - http://www.scopus.com/inward/record.url?scp=85113741747&partnerID=8YFLogxK
U2 - 10.1007/s11033-021-06601-9
DO - 10.1007/s11033-021-06601-9
M3 - Artículo
C2 - 34426903
AN - SCOPUS:85113741747
SN - 0301-4851
VL - 48
SP - 7007
EP - 7012
JO - Molecular Biology Reports
JF - Molecular Biology Reports
IS - 10
ER -