TY - JOUR
T1 - Neutrophil extracellular traps are induced by Mycobacterium tuberculosis
AU - Ramos-Kichik, Victoria
AU - Mondragón-Flores, Ricardo
AU - Mondragón-Castelán, Mónica
AU - Gonzalez-Pozos, Sirenia
AU - Muñiz-Hernandez, Sae
AU - Rojas-Espinosa, Oscar
AU - Chacón-Salinas, Rommel
AU - Estrada-Parra, Sergio
AU - Estrada-García, Iris
N1 - Funding Information:
We would like to thank Arturo Zychlinsky for kindly providing the neutrophil elastase and the H2A-H2B-DNA complex monoclonal antibodies and also for technical advice and critical reviewing of this work. We are also grateful to other members of the Max-Planck Institute for Infection Biology in Berlin, Germany: Catherine Chaput, Constantin Urban, Volker Brinkmann, Juana de Diego and Cornelia Heinz. This work was supported by CONACYT Grant No.42512 and SIP 20082200.
PY - 2009/1
Y1 - 2009/1
N2 - Due to the intracellular nature of mycobacterial infections, little attention has been paid to the possible extracellular role that neutrophils might play in tuberculosis. The recent discovery of neutrophil extracellular traps (NETs), composed of DNA and antimicrobial proteins,1 introduces a new perspective to our understanding of the mechanism used by the innate immune system to contain and kill microorganisms. In this study, we tested in vitro whether Mycobacterium tuberculosis, an intracellular pathogen, can induce NETs formation and if this newly discovered mechanism is involved in a control response during mycobacterial infection. We found that two different genotypes of M. tuberculosis exerted, in vitro, a cytotoxic effect and induced subcellular changes on infected neutrophils, leading to NETs formation in a time dependent manner. NETs trapped mycobacteria but were unable to kill them. NETs formation induced by M. tuberculosis could help understand the early stages of mycobacterial pathogenesis.
AB - Due to the intracellular nature of mycobacterial infections, little attention has been paid to the possible extracellular role that neutrophils might play in tuberculosis. The recent discovery of neutrophil extracellular traps (NETs), composed of DNA and antimicrobial proteins,1 introduces a new perspective to our understanding of the mechanism used by the innate immune system to contain and kill microorganisms. In this study, we tested in vitro whether Mycobacterium tuberculosis, an intracellular pathogen, can induce NETs formation and if this newly discovered mechanism is involved in a control response during mycobacterial infection. We found that two different genotypes of M. tuberculosis exerted, in vitro, a cytotoxic effect and induced subcellular changes on infected neutrophils, leading to NETs formation in a time dependent manner. NETs trapped mycobacteria but were unable to kill them. NETs formation induced by M. tuberculosis could help understand the early stages of mycobacterial pathogenesis.
KW - M. canettii
KW - M. tuberculosis genotypes
KW - NETs
KW - Neutrophils
KW - ROS
UR - http://www.scopus.com/inward/record.url?scp=58149116515&partnerID=8YFLogxK
U2 - 10.1016/j.tube.2008.09.009
DO - 10.1016/j.tube.2008.09.009
M3 - Artículo
C2 - 19056316
SN - 1472-9792
VL - 89
SP - 29
EP - 37
JO - Tuberculosis
JF - Tuberculosis
IS - 1
ER -