TY - JOUR
T1 - Neuronal Nitric Oxide Synthase in Cultured Cerebellar Bergmann Glia
T2 - Glutamate-Dependent Regulation
AU - Tiburcio-Félix, Reynaldo
AU - Cisneros, Bulmaro
AU - Hernández-Kelly, Luisa C.R.
AU - Hernández-Contreras, María A.
AU - Luna-Herrera, Julieta
AU - Rea-Hernández, Ismael
AU - Jiménez-Aguilar, Rosalinda
AU - Olivares-Bañuelos, Tatiana N.
AU - Ortega, Arturo
N1 - Publisher Copyright:
© 2019 American Chemical Society.
PY - 2019/6/19
Y1 - 2019/6/19
N2 - Glutamate exerts its actions through the activation of membrane receptors expressed in neurons and glia cells. The signaling properties of glutamate transporters have been characterized recently, suggesting a complex array of signaling transactions triggered by presynaptic released glutamate. In the cerebellar molecular layer, glutamatergic synapses are surrounded by Bergmann glia cells, compulsory participants of glutamate turnover and supply to neurons. Since a glutamate-dependent increase in cGMP levels has been described in these cells and the nitric oxide-cGMP signaling cascade increases their glutamate uptake activity, we describe here the Bergmann glia expression of neuronal nitric oxide synthetase. An augmentation of neuronal nitric oxide synthase was found upon glutamate exposure. This effect is mediated by glutamate transporters and is related to an increase in the stability of the enzyme. These results strengthen the notion of a complex regulation of glial glutamate uptake that supports neuronal glutamate signaling.
AB - Glutamate exerts its actions through the activation of membrane receptors expressed in neurons and glia cells. The signaling properties of glutamate transporters have been characterized recently, suggesting a complex array of signaling transactions triggered by presynaptic released glutamate. In the cerebellar molecular layer, glutamatergic synapses are surrounded by Bergmann glia cells, compulsory participants of glutamate turnover and supply to neurons. Since a glutamate-dependent increase in cGMP levels has been described in these cells and the nitric oxide-cGMP signaling cascade increases their glutamate uptake activity, we describe here the Bergmann glia expression of neuronal nitric oxide synthetase. An augmentation of neuronal nitric oxide synthase was found upon glutamate exposure. This effect is mediated by glutamate transporters and is related to an increase in the stability of the enzyme. These results strengthen the notion of a complex regulation of glial glutamate uptake that supports neuronal glutamate signaling.
KW - Bergmann glia
KW - cGMP
KW - glutamate transporters
KW - neuronal nitric oxide synthetase
KW - protein stability
UR - http://www.scopus.com/inward/record.url?scp=85067509007&partnerID=8YFLogxK
U2 - 10.1021/acschemneuro.8b00656
DO - 10.1021/acschemneuro.8b00656
M3 - Artículo
C2 - 31091406
SN - 1948-7193
VL - 10
SP - 2668
EP - 2675
JO - ACS Chemical Neuroscience
JF - ACS Chemical Neuroscience
IS - 6
ER -