TY - JOUR
T1 - Nematicidal Effect of Shiitake (Lentinula edodes) Extracts against Haemonchus contortus
AU - Pineda-Alegría, Jesús Antonio
AU - Sánchez, José E.
AU - Ventura-Zapata, Elsa
AU - González-Cortazar, Manases
AU - Aguilar-Marcelino, Liliana
N1 - Publisher Copyright:
© Copyright 2021, Mary Ann Liebert, Inc., publishers, and Korean Society of Food Science and Nutrition 2021.
PY - 2021/9
Y1 - 2021/9
N2 - During the previous decades, the indiscriminate use of anthelmintics for the control of gastrointestinal nematodes has generated anthelmintic resistance in different parts of the world. It is necessary to search for new sustainable control alternatives, such as the use of extracts from plants and edible mushrooms. Therefore, the aim of the present study was to evaluate the nematicidal activity of extracts and fractions of the edible mushroom Lentinula edodes against Haemonchus contortus eggs and infective larvae. The basidiomata of L. edodes ECS-401 were provided by the Tropical Fungi Laboratory of El Colegio de la Frontera Sur and were extracted with ethyl acetate, methanol, and water. Fractionation of the most active extract was carried out by open column chromatography. The bioassays were performed in 96-well microtiter plates using 100 eggs/larvae, a final volume of 100 μL, and different concentrations of extracts/fractions (n = 4). Bioassay readings were taken at 48 h for egg hatching inhibition (EHI) and at 24, 48 and 72 h for larval mortality (LM). The ethyl acetate extract exhibited the highest percentage of EHI (100%). For the LM bioassay, the aqueous extract was the most active (69%), but its fractions did not show larvicidal activity. The chemical profile of the aqueous extract was analyzed by high-performance liquid chromatography, which showed the presence of phenols, flavonoids, and terpenes. L. edodes extracts showed ovicidal and/or larvicidal activity.
AB - During the previous decades, the indiscriminate use of anthelmintics for the control of gastrointestinal nematodes has generated anthelmintic resistance in different parts of the world. It is necessary to search for new sustainable control alternatives, such as the use of extracts from plants and edible mushrooms. Therefore, the aim of the present study was to evaluate the nematicidal activity of extracts and fractions of the edible mushroom Lentinula edodes against Haemonchus contortus eggs and infective larvae. The basidiomata of L. edodes ECS-401 were provided by the Tropical Fungi Laboratory of El Colegio de la Frontera Sur and were extracted with ethyl acetate, methanol, and water. Fractionation of the most active extract was carried out by open column chromatography. The bioassays were performed in 96-well microtiter plates using 100 eggs/larvae, a final volume of 100 μL, and different concentrations of extracts/fractions (n = 4). Bioassay readings were taken at 48 h for egg hatching inhibition (EHI) and at 24, 48 and 72 h for larval mortality (LM). The ethyl acetate extract exhibited the highest percentage of EHI (100%). For the LM bioassay, the aqueous extract was the most active (69%), but its fractions did not show larvicidal activity. The chemical profile of the aqueous extract was analyzed by high-performance liquid chromatography, which showed the presence of phenols, flavonoids, and terpenes. L. edodes extracts showed ovicidal and/or larvicidal activity.
KW - Haemonchus contortus
KW - Lentinula edodes
KW - Shiitake
KW - bioactive compounds
KW - extracts
UR - http://www.scopus.com/inward/record.url?scp=85115217742&partnerID=8YFLogxK
U2 - 10.1089/jmf.2020.0168
DO - 10.1089/jmf.2020.0168
M3 - Artículo
C2 - 33733862
AN - SCOPUS:85115217742
SN - 1096-620X
VL - 24
SP - 953
EP - 959
JO - Journal of Medicinal Food
JF - Journal of Medicinal Food
IS - 9
ER -