Multiplex PCR for detection of three plasmid-borne genes of enteroaggregative Escherichia coli strains

Jorge F. Cerna, James P. Nataro, Teresa Estrada-Garcia

Research output: Contribution to journalArticlepeer-review

132 Scopus citations

Abstract

We developed a novel multiplex PCR assay for enteroaggregative Escherichia coli (EAEC) detection, by using three plasmid-borne genes (the aggregative adherence [AA] probe, aap, and aggR). One or more of the loci were detected in 24 (86%) of 28 patient isolates analyzed. The multiplex PCR assay is a fast, convenient, and sensitive molecular test to detect EAEC.

Original languageEnglish
Pages (from-to)2138-2140
Number of pages3
JournalJournal of Clinical Microbiology
Volume41
Issue number5
DOIs
StatePublished - 1 May 2003
Externally publishedYes

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