Mouse neutrophils release extracellular traps in response to Naegleria fowleri

Maria Maricela Carrasco-Yepez, Arturo Contis-Montes de Oca, Rafael Campos-Rodriguez, Diana Falcon-Acosta, Judith Pacheco-Yepez, Itzel Berenice Rodriguez-Mera, Patricia Bonilla-Lemus, Erika Rosales-Cruz, Israel Lopez-Reyes, Saul Rojas-Hernandez

Research output: Contribution to journalArticle

Abstract

© 2018 John Wiley & Sons Ltd Naegleria fowleri is a free-living amoeba, which is able to infect humans through the nasal mucosa causing a disease in the central nervous system known as primary amoebic meningoencephalitis (PAM). Polymorphonuclear cells (PMNs) play a critical role in the early phase of N fowleri infection. Recently, a new biological defence mechanism called neutrophil extracellular traps (NETs) has been attracting attention. These structures represent an important strategy to immobilize and kill invading microorganisms. In this work, we evaluate the capacity of N fowleri to induce the NETs release by PMNs cells in mice in vitro and in vivo. In vitro: Neutrophils from bone marrow were cocultured with N fowleri trophozoites. In vivo: we employed a mouse model of PAM. We evaluated DNA, histone and myeloperoxidase (MPO) and the formation of NETs by confocal microscopy. Our results showed N fowleri induce both NETs and MPO release by PMNs cells in mice after trophozoite exposure, which increased through time, in vitro and in vivo. These results demonstrate that NETs are somehow associated with the amoebas. We suggest PMNs release their traps trying to avoid N fowleri attachment at the apical side of the nasal epithelium.
Original languageAmerican English
JournalParasite Immunology
DOIs
StatePublished - 1 Feb 2019

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Naegleria fowleri
Trophozoites
Amoeba
Nasal Mucosa
Peroxidase
Central Nervous System Diseases
Confocal Microscopy
Histones
Neutrophils
Bone Marrow
Extracellular Traps
DNA
Infection
In Vitro Techniques

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Carrasco-Yepez, Maria Maricela ; Contis-Montes de Oca, Arturo ; Campos-Rodriguez, Rafael ; Falcon-Acosta, Diana ; Pacheco-Yepez, Judith ; Rodriguez-Mera, Itzel Berenice ; Bonilla-Lemus, Patricia ; Rosales-Cruz, Erika ; Lopez-Reyes, Israel ; Rojas-Hernandez, Saul. / Mouse neutrophils release extracellular traps in response to Naegleria fowleri. In: Parasite Immunology. 2019.
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Mouse neutrophils release extracellular traps in response to Naegleria fowleri. / Carrasco-Yepez, Maria Maricela; Contis-Montes de Oca, Arturo; Campos-Rodriguez, Rafael; Falcon-Acosta, Diana; Pacheco-Yepez, Judith; Rodriguez-Mera, Itzel Berenice; Bonilla-Lemus, Patricia; Rosales-Cruz, Erika; Lopez-Reyes, Israel; Rojas-Hernandez, Saul.

In: Parasite Immunology, 01.02.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Mouse neutrophils release extracellular traps in response to Naegleria fowleri

AU - Carrasco-Yepez, Maria Maricela

AU - Contis-Montes de Oca, Arturo

AU - Campos-Rodriguez, Rafael

AU - Falcon-Acosta, Diana

AU - Pacheco-Yepez, Judith

AU - Rodriguez-Mera, Itzel Berenice

AU - Bonilla-Lemus, Patricia

AU - Rosales-Cruz, Erika

AU - Lopez-Reyes, Israel

AU - Rojas-Hernandez, Saul

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N2 - © 2018 John Wiley & Sons Ltd Naegleria fowleri is a free-living amoeba, which is able to infect humans through the nasal mucosa causing a disease in the central nervous system known as primary amoebic meningoencephalitis (PAM). Polymorphonuclear cells (PMNs) play a critical role in the early phase of N fowleri infection. Recently, a new biological defence mechanism called neutrophil extracellular traps (NETs) has been attracting attention. These structures represent an important strategy to immobilize and kill invading microorganisms. In this work, we evaluate the capacity of N fowleri to induce the NETs release by PMNs cells in mice in vitro and in vivo. In vitro: Neutrophils from bone marrow were cocultured with N fowleri trophozoites. In vivo: we employed a mouse model of PAM. We evaluated DNA, histone and myeloperoxidase (MPO) and the formation of NETs by confocal microscopy. Our results showed N fowleri induce both NETs and MPO release by PMNs cells in mice after trophozoite exposure, which increased through time, in vitro and in vivo. These results demonstrate that NETs are somehow associated with the amoebas. We suggest PMNs release their traps trying to avoid N fowleri attachment at the apical side of the nasal epithelium.

AB - © 2018 John Wiley & Sons Ltd Naegleria fowleri is a free-living amoeba, which is able to infect humans through the nasal mucosa causing a disease in the central nervous system known as primary amoebic meningoencephalitis (PAM). Polymorphonuclear cells (PMNs) play a critical role in the early phase of N fowleri infection. Recently, a new biological defence mechanism called neutrophil extracellular traps (NETs) has been attracting attention. These structures represent an important strategy to immobilize and kill invading microorganisms. In this work, we evaluate the capacity of N fowleri to induce the NETs release by PMNs cells in mice in vitro and in vivo. In vitro: Neutrophils from bone marrow were cocultured with N fowleri trophozoites. In vivo: we employed a mouse model of PAM. We evaluated DNA, histone and myeloperoxidase (MPO) and the formation of NETs by confocal microscopy. Our results showed N fowleri induce both NETs and MPO release by PMNs cells in mice after trophozoite exposure, which increased through time, in vitro and in vivo. These results demonstrate that NETs are somehow associated with the amoebas. We suggest PMNs release their traps trying to avoid N fowleri attachment at the apical side of the nasal epithelium.

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