TY - JOUR
T1 - Mouse models for the study of postnatal cardiac hypertrophy
AU - Del Olmo-Turrubiarte, A.
AU - Calzada-Torres, A.
AU - Díaz-Rosas, G.
AU - Palma-Lara, I.
AU - Sánchez-Urbina, R.
AU - Balderrábano-Saucedo, N. A.
AU - González-Márquez, H.
AU - Garcia-Alonso, P.
AU - Contreras-Ramos, A.
N1 - Publisher Copyright:
© 2015 The Authors. Published by Elsevier Ireland Ltd.
PY - 2015/6/1
Y1 - 2015/6/1
N2 - The main objective of this study was to create a postnatal model for cardiac hypertrophy (CH), in order to explain the mechanisms that are present in childhood cardiac hypertrophy. Five days after implantation, intraperitoneal (IP) isoproterenol (ISO) was injected for 7. days to pregnant female mice. The fetuses were obtained at 15, 17 and 19. dpc from both groups, also newborns (NB), neonates (7-15. days) and young adults (6. weeks of age). Histopathological exams were done on the hearts. Immunohistochemistry and western blot demonstrated GATA4 and PCNA protein expression, qPCR real time the mRNA of adrenergic receptors (α-AR and β-AR), alpha and beta myosins (α-MHC, β-MHC) and GATA4. After the administration of ISO, there was no change in the number of offsprings. We observed significant structural changes in the size of the offspring hearts. Morphometric analysis revealed an increase in the size of the left ventricular wall and interventricular septum (IVS). Histopathological analysis demonstrated loss of cellular compaction and presence of left ventricular small fibrous foci after birth. Adrenergic receptors might be responsible for changing a physiological into a pathological hypertrophy. However GATA4 seemed to be the determining factor in the pathology. A new animal model was established for the study of pathologic CH in early postnatal stages.
AB - The main objective of this study was to create a postnatal model for cardiac hypertrophy (CH), in order to explain the mechanisms that are present in childhood cardiac hypertrophy. Five days after implantation, intraperitoneal (IP) isoproterenol (ISO) was injected for 7. days to pregnant female mice. The fetuses were obtained at 15, 17 and 19. dpc from both groups, also newborns (NB), neonates (7-15. days) and young adults (6. weeks of age). Histopathological exams were done on the hearts. Immunohistochemistry and western blot demonstrated GATA4 and PCNA protein expression, qPCR real time the mRNA of adrenergic receptors (α-AR and β-AR), alpha and beta myosins (α-MHC, β-MHC) and GATA4. After the administration of ISO, there was no change in the number of offsprings. We observed significant structural changes in the size of the offspring hearts. Morphometric analysis revealed an increase in the size of the left ventricular wall and interventricular septum (IVS). Histopathological analysis demonstrated loss of cellular compaction and presence of left ventricular small fibrous foci after birth. Adrenergic receptors might be responsible for changing a physiological into a pathological hypertrophy. However GATA4 seemed to be the determining factor in the pathology. A new animal model was established for the study of pathologic CH in early postnatal stages.
KW - Fetal cardiac hypertrophy
KW - GATA4
KW - Isoproterenol
KW - α-MHC
KW - β-MHC
UR - http://www.scopus.com/inward/record.url?scp=84930649656&partnerID=8YFLogxK
U2 - 10.1016/j.ijcha.2015.02.005
DO - 10.1016/j.ijcha.2015.02.005
M3 - Artículo
SN - 2352-9067
VL - 7
SP - 131
EP - 140
JO - IJC Heart and Vasculature
JF - IJC Heart and Vasculature
ER -