TY - JOUR
T1 - Mosquito cells persistently infected with dengue virus produce viral particles with host-dependent replication
AU - Reyes-Ruiz, José Manuel
AU - Osuna-Ramos, Juan Fidel
AU - Bautista-Carbajal, Patricia
AU - Jaworski, Elizabeth
AU - Soto-Acosta, Rubén
AU - Cervantes-Salazar, Margot
AU - Angel-Ambrocio, Antonio H.
AU - Castillo-Munguía, Juan Pablo
AU - Chávez-Munguía, Bibiana
AU - De Nova-Ocampo, Mónica
AU - Routh, Andrew
AU - del Ángel, Rosa María
AU - Salas-Benito, Juan Santiago
N1 - Publisher Copyright:
© 2019 Elsevier Inc.
PY - 2019/5
Y1 - 2019/5
N2 - Dengue viruses (DENV) are important arboviruses that can establish a persistent infection in its mosquito vector Aedes. Mosquitoes have a short lifetime in nature which makes trying to study the processes that take place during persistent viral infections in vivo. Therefore, C6/36 cells have been used to study this type of infection. C6/36 cells persistently infected with DENV 2 produce virions that cannot infect BHK -21 cells. We hypothesized that the following passages in mosquito cells have a deleterious impact on DENV fitness in vertebrate cells. Here, we demonstrated that the viral particles released from persistently infected cells were infectious to mosquito but not to vertebrate cells. This host restriction occurs at the replication level and is associated with several mutations in the DENV genome. In summary, our findings provide new information about viral replication fitness in a host-dependent manner.
AB - Dengue viruses (DENV) are important arboviruses that can establish a persistent infection in its mosquito vector Aedes. Mosquitoes have a short lifetime in nature which makes trying to study the processes that take place during persistent viral infections in vivo. Therefore, C6/36 cells have been used to study this type of infection. C6/36 cells persistently infected with DENV 2 produce virions that cannot infect BHK -21 cells. We hypothesized that the following passages in mosquito cells have a deleterious impact on DENV fitness in vertebrate cells. Here, we demonstrated that the viral particles released from persistently infected cells were infectious to mosquito but not to vertebrate cells. This host restriction occurs at the replication level and is associated with several mutations in the DENV genome. In summary, our findings provide new information about viral replication fitness in a host-dependent manner.
KW - Dengue viruses
KW - Host adaptation
KW - Mosquito cells
KW - Persistent viral infection
KW - Viral fitness
UR - http://www.scopus.com/inward/record.url?scp=85062725676&partnerID=8YFLogxK
U2 - 10.1016/j.virol.2019.02.018
DO - 10.1016/j.virol.2019.02.018
M3 - Artículo
C2 - 30844508
SN - 0042-6822
VL - 531
SP - 1
EP - 18
JO - Virology
JF - Virology
ER -
