TY - JOUR
T1 - Morphological description of the early events during the invasion of acanthamoeba castellanii trophozoites in a murine model of skin irradiated under uv-b light
AU - Hernández-Jasso, Mariana
AU - Hernández-Martínez, Dolores
AU - Ávila-Acevedo, José Guillermo
AU - Benítez-Flores, José Del Carmen
AU - Gallegos-Hernández, Isis Amara
AU - García-Bores, Ana María
AU - Espinoza-González, Adriana Montserrat
AU - Villamar-Duque, Tomás Ernesto
AU - Castelan-Ramírez, Ismael
AU - González-Valle, María Del Rosario
AU - Omaña-Molina, Maritza
N1 - Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2020/10
Y1 - 2020/10
N2 - Skin infections have been associated with Acanthamoeba, nevertheless the events during skin invasion and UV-B light effects on it are unknown. The early morphological events of Acanthamoeba castellanii skin invasion are shown in SKH-1 mice that were chronically UV-B light irradiated. Mice that developed skin lesions (group 1) were topical and intradermally inoculated with A. castellanii trophozoites and sacrificed 48 h or 18 days later. Mice that showed no skin lesions (group 2) were intradermally inoculated and sacrificed 24, 48 or 72 h later. Mice ventral areas were considered controls with and without trophozoites intradermally inoculated. Skin samples were processed by histological and immunohistochemistry techniques. In group 1, trophozoites were immunolocalized in dermal areas, hair cysts, sebaceous glands, and blood vessels, and collagen degradation was observed. One of these mice shown trophozoites in the spleen, liver, and brain. In group 2, few trophozoites nearby collagenolytic activity zones were observed. In control samples, nor histological damage and no trophozoites were observed. Adherence and collagenolytic activity by A. castellanii were corroborated in vitro. We can infer that UV-B light irradiated skin could favor A. castellanii invasiveness causing damage in sites as far away as the brain, confirming the invasive capacity and pathogenic potential of these amphizoic amoebae.
AB - Skin infections have been associated with Acanthamoeba, nevertheless the events during skin invasion and UV-B light effects on it are unknown. The early morphological events of Acanthamoeba castellanii skin invasion are shown in SKH-1 mice that were chronically UV-B light irradiated. Mice that developed skin lesions (group 1) were topical and intradermally inoculated with A. castellanii trophozoites and sacrificed 48 h or 18 days later. Mice that showed no skin lesions (group 2) were intradermally inoculated and sacrificed 24, 48 or 72 h later. Mice ventral areas were considered controls with and without trophozoites intradermally inoculated. Skin samples were processed by histological and immunohistochemistry techniques. In group 1, trophozoites were immunolocalized in dermal areas, hair cysts, sebaceous glands, and blood vessels, and collagen degradation was observed. One of these mice shown trophozoites in the spleen, liver, and brain. In group 2, few trophozoites nearby collagenolytic activity zones were observed. In control samples, nor histological damage and no trophozoites were observed. Adherence and collagenolytic activity by A. castellanii were corroborated in vitro. We can infer that UV-B light irradiated skin could favor A. castellanii invasiveness causing damage in sites as far away as the brain, confirming the invasive capacity and pathogenic potential of these amphizoic amoebae.
KW - Acanthamoeba castellanii
KW - Murine model
KW - Pathogenic mechanism
KW - Skin invasion
UR - http://www.scopus.com/inward/record.url?scp=85091667243&partnerID=8YFLogxK
U2 - 10.3390/pathogens9100794
DO - 10.3390/pathogens9100794
M3 - Artículo
AN - SCOPUS:85091667243
SN - 2076-0817
VL - 9
SP - 1
EP - 15
JO - Pathogens
JF - Pathogens
IS - 10
M1 - 794
ER -