TY - JOUR
T1 - Morphological changes, chitinolytic enzymes and hydrophobin-like proteins as responses of Lecanicillium lecanii during growth with hydrocarbon
AU - Rocha-Pino, Zaizy
AU - Del Carmen Marín-Cervantes, María
AU - Martínez-Archundia, Marlet
AU - Soriano-Blancas, Elizabeth
AU - Revah, Sergio
AU - Shirai, Keiko
N1 - Funding Information:
Acknowledgments The authors would like to thank SEP-CONA-CYT (No. 105628) for research funding and scholarship grants to Miss Rocha-Pino and Miss Marín-Cervantes. Dr. José Sepulveda is greatly acknowledged for his invaluable assistance in the SEM studies.
PY - 2013/5
Y1 - 2013/5
N2 - Lecanicillium lecanii, Verticillium chlamydosporium, V. fungicola var flavidum and Beauveria bassiana were evaluated on their growth with pure n-hexane, toluene and n-hexane:toluene 17:83 (v:v) mixture. Another set of treatments were conducted with colloidal chitin as additional carbon source. All the strains of Lecanicillium were able to grow using hydrocarbons with or without the addition of chitin, although the presence of hydrocarbons showed significant inhibition evidenced by measured biomass, radial growth and microscopic analyses. Degradation of n-hexane ranged within 43 and 62 % and it was higher than that with toluene. The strains L460, L157 and L2149, which presented the highest growth, were further selected for determinations of hydrocarbon consumptions in microcosms. Strain L157 showed the highest consumption of n-hexane (55.6 %) and toluene (52.9 %) as sole carbon source and it also displayed activities of endochitinases, N-acetylhexosaminidase and production of hydrophobins class I and II.
AB - Lecanicillium lecanii, Verticillium chlamydosporium, V. fungicola var flavidum and Beauveria bassiana were evaluated on their growth with pure n-hexane, toluene and n-hexane:toluene 17:83 (v:v) mixture. Another set of treatments were conducted with colloidal chitin as additional carbon source. All the strains of Lecanicillium were able to grow using hydrocarbons with or without the addition of chitin, although the presence of hydrocarbons showed significant inhibition evidenced by measured biomass, radial growth and microscopic analyses. Degradation of n-hexane ranged within 43 and 62 % and it was higher than that with toluene. The strains L460, L157 and L2149, which presented the highest growth, were further selected for determinations of hydrocarbon consumptions in microcosms. Strain L157 showed the highest consumption of n-hexane (55.6 %) and toluene (52.9 %) as sole carbon source and it also displayed activities of endochitinases, N-acetylhexosaminidase and production of hydrophobins class I and II.
KW - Chitinases
KW - Entomopathogenic fungi
KW - Hydrocarbons
KW - Hydrophobins
UR - http://www.scopus.com/inward/record.url?scp=84877921046&partnerID=8YFLogxK
U2 - 10.1007/s00449-012-0808-z
DO - 10.1007/s00449-012-0808-z
M3 - Artículo
C2 - 22926786
SN - 1615-7591
VL - 36
SP - 531
EP - 539
JO - Bioprocess and Biosystems Engineering
JF - Bioprocess and Biosystems Engineering
IS - 5
ER -