TY - JOUR
T1 - Molecular typification of Escherichia coli from community-acquired urinary tract infections in Mexico
AU - Belmont-Monroy, Laura
AU - Ribas-Aparicio, Rosa María
AU - González-Villalobos, Edgar
AU - Pérez-Ramos, José Alejandro
AU - Aparicio-Ozores, Gerardo
AU - Eslava-Campos, Carlos A.
AU - Hernández-Chiñas, Ulises
AU - Aquino-Andrade, Alejandra
AU - Balcázar, José Luis
AU - Molina-López, José
N1 - Publisher Copyright:
© 2022 Elsevier Ltd and International Society of Antimicrobial Chemotherapy
PY - 2022/10
Y1 - 2022/10
N2 - One hundred and five uropathogenic Escherichia coli (UPEC) strains from patients with community-acquired urinary tract infections were characterized according to phylogenetic group, virulence factors, serogroup, antibiotic resistance, and genotype. The pathogenic phylogenetic groups (B2, D, and F) were found in 71.4% of the tested strains. Among them, the main uropathogenic serogroups were O8, O25, and O75, in which 97.1% of the strains had a multidrug-resistant profile. Sixteen virulence genes were analysed using a combination of polymerase chain reaction (PCR) assays, with the fimH, irp-2, iutA, aer, iucC, PAI, sat, iroN, usp, and cnf1 genes being mainly found in pathogenic phylogroups. The E. coli O25b-ST131 clone was identified in 32% of the strains assigned to the pathogenic phylogroup B2. These findings demonstrate that virulence genes encoding adhesin components, iron-acquisition systems, toxins, and pathogenicity-associated islands were highly prevalent among the pathogenic phylogroup of UPEC strains.
AB - One hundred and five uropathogenic Escherichia coli (UPEC) strains from patients with community-acquired urinary tract infections were characterized according to phylogenetic group, virulence factors, serogroup, antibiotic resistance, and genotype. The pathogenic phylogenetic groups (B2, D, and F) were found in 71.4% of the tested strains. Among them, the main uropathogenic serogroups were O8, O25, and O75, in which 97.1% of the strains had a multidrug-resistant profile. Sixteen virulence genes were analysed using a combination of polymerase chain reaction (PCR) assays, with the fimH, irp-2, iutA, aer, iucC, PAI, sat, iroN, usp, and cnf1 genes being mainly found in pathogenic phylogroups. The E. coli O25b-ST131 clone was identified in 32% of the strains assigned to the pathogenic phylogroup B2. These findings demonstrate that virulence genes encoding adhesin components, iron-acquisition systems, toxins, and pathogenicity-associated islands were highly prevalent among the pathogenic phylogroup of UPEC strains.
KW - antibiotic resistance
KW - community-acquired urinary tract infection
KW - new Clermont phylotyping
KW - uropathogenic E. coli
KW - virulence factors
UR - http://www.scopus.com/inward/record.url?scp=85138178077&partnerID=8YFLogxK
U2 - 10.1016/j.ijantimicag.2022.106667
DO - 10.1016/j.ijantimicag.2022.106667
M3 - Artículo
C2 - 36038094
AN - SCOPUS:85138178077
SN - 0924-8579
VL - 60
JO - International Journal of Antimicrobial Agents
JF - International Journal of Antimicrobial Agents
IS - 4
M1 - 106667
ER -
