TY - JOUR
T1 - Molecular studies of Onchocerca volvulus isolates from Mexico
AU - Ramírez-Ramírez, Alicia
AU - Sánchez-Tejeda, Gustavo
AU - Méndez-Galván, Jorge
AU - Unnasch, Thomas R.
AU - Monroy-Ostria, Amalia
N1 - Funding Information:
This work was supported by CGPI, IPN (CGPI 32.05). Alicia Ramírez-Ramírez, is sponsored by CONACyT, México. Amalia Monroy-Ostria is sponsored by COFAA and EDI, IPN, México.
PY - 2006/5
Y1 - 2006/5
N2 - DNA from Onchocerca volvulus from Oaxaca and Chiapas, Mexico were used as templates to amplify members of the O-150 Onchocerca specific repeat sequence family. The resulting PCR amplicons all hybridized with OVS2, an oligonucleotide that has been previously shown to recognize amplicons derived from O. volvulus with 100% sensitivity. However, when PCR products amplified from the O. volvulus specific plasmid pOVS134 were used as a probe, most samples did not hybridize. Similarly, when PCR products amplified from DNA isolated from adult O. volvulus from Oaxaca were used as a probe, amplicons from adult worms from both Oaxaca and Chiapas were recognized, but PCR products from infected black flies from Chiapas were not recognized. Amplicons derived from an adult worm from Chiapas hybridized with PCR products produced from adult parasites from both Oaxaca and Chiapas and to PCR products derived from the DNA of infected black flies from Chiapas. These data, when taken together, suggest that differences exist among the repeat sequence populations of parasites from Oaxaca and Chiapas in Mexico, suggesting that the O-150 repeat sequence family may be a useful tool for biogeographic studies of O. volvulus in the Americas.
AB - DNA from Onchocerca volvulus from Oaxaca and Chiapas, Mexico were used as templates to amplify members of the O-150 Onchocerca specific repeat sequence family. The resulting PCR amplicons all hybridized with OVS2, an oligonucleotide that has been previously shown to recognize amplicons derived from O. volvulus with 100% sensitivity. However, when PCR products amplified from the O. volvulus specific plasmid pOVS134 were used as a probe, most samples did not hybridize. Similarly, when PCR products amplified from DNA isolated from adult O. volvulus from Oaxaca were used as a probe, amplicons from adult worms from both Oaxaca and Chiapas were recognized, but PCR products from infected black flies from Chiapas were not recognized. Amplicons derived from an adult worm from Chiapas hybridized with PCR products produced from adult parasites from both Oaxaca and Chiapas and to PCR products derived from the DNA of infected black flies from Chiapas. These data, when taken together, suggest that differences exist among the repeat sequence populations of parasites from Oaxaca and Chiapas in Mexico, suggesting that the O-150 repeat sequence family may be a useful tool for biogeographic studies of O. volvulus in the Americas.
KW - Geographical diversity
KW - Isolate
KW - Mexico
KW - Molecular probe
KW - Onchocerca volvulus
UR - http://www.scopus.com/inward/record.url?scp=33645558577&partnerID=8YFLogxK
U2 - 10.1016/j.meegid.2005.03.001
DO - 10.1016/j.meegid.2005.03.001
M3 - Artículo
SN - 1567-1348
VL - 6
SP - 171
EP - 176
JO - Infection, Genetics and Evolution
JF - Infection, Genetics and Evolution
IS - 3
ER -