Modified penicillin acylase signal peptide allows the periplasmic production of soluble human interferon-γ but not of soluble human interleukin-2 by the Tat pathway in Escherichia coli

E. Medina-Rivero; V. E. Balderas-Hernández; L. G. Ordoñez-Acevedo; L. M.T. Paz-Maldonado; A. P. Barba-De La Rosa; A. De León-Rodríguez

doi:10.1007/s10529-007-9395-5

Modified penicillin acylase signal peptide allows the periplasmic production of soluble human interferon-γ but not of soluble human interleukin-2 by the Tat pathway in Escherichia coli

E. Medina-Rivero, V. E. Balderas-Hernández, L. G. Ordoñez-Acevedo, L. M.T. Paz-Maldonado, A. P. Barba-De La Rosa, A. De León-Rodríguez

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8 Scopus citations

Abstract

Production of periplasmic human interferon-γ (hINF-γ) and human interleukin-2 (hIL-2) by the Tat translocation pathway in Escherichia coli BL21-SI was evaluated. The expression was obtained using the pEMR vector which contains the Tat-dependent modified penicillin acylase signal peptide (mSPpac) driven by the T7 promoter. The mSPpac-hINF-γ was processed and the protein was transported to periplasm. Up to 30.1% of hINF-γ was found in the periplasmic soluble fraction, whereas only 15% of the mSPpac-hIL-2 was processed, but hIL-2 was not found in the periplasmic soluble fraction.

Original language	English
Pages (from-to)	1369-1374
Number of pages	6
Journal	Biotechnology Letters
Volume	29
Issue number	9
DOIs	https://doi.org/10.1007/s10529-007-9395-5
State	Published - Sep 2007
Externally published	Yes

Keywords

Periplasm
Signal peptide
Tat pathway
Therapeutic protein

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Medina-Rivero, E., Balderas-Hernández, V. E., Ordoñez-Acevedo, L. G., Paz-Maldonado, L. M. T., Barba-De La Rosa, A. P., & De León-Rodríguez, A. (2007). Modified penicillin acylase signal peptide allows the periplasmic production of soluble human interferon-γ but not of soluble human interleukin-2 by the Tat pathway in Escherichia coli. Biotechnology Letters, 29(9), 1369-1374. https://doi.org/10.1007/s10529-007-9395-5

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title = "Modified penicillin acylase signal peptide allows the periplasmic production of soluble human interferon-γ but not of soluble human interleukin-2 by the Tat pathway in Escherichia coli",

abstract = "Production of periplasmic human interferon-γ (hINF-γ) and human interleukin-2 (hIL-2) by the Tat translocation pathway in Escherichia coli BL21-SI was evaluated. The expression was obtained using the pEMR vector which contains the Tat-dependent modified penicillin acylase signal peptide (mSPpac) driven by the T7 promoter. The mSPpac-hINF-γ was processed and the protein was transported to periplasm. Up to 30.1% of hINF-γ was found in the periplasmic soluble fraction, whereas only 15% of the mSPpac-hIL-2 was processed, but hIL-2 was not found in the periplasmic soluble fraction.",

keywords = "Periplasm, Signal peptide, Tat pathway, Therapeutic protein",

author = "E. Medina-Rivero and Balderas-Hern{\'a}ndez, {V. E.} and Ordo{\~n}ez-Acevedo, {L. G.} and Paz-Maldonado, {L. M.T.} and {Barba-De La Rosa}, {A. P.} and {De Le{\'o}n-Rodr{\'i}guez}, A.",

note = "Funding Information: Acknowledgments This work was partially supported by CONACyT Grant J39639-Q and a FOMIX grant FMSLP-2002–4100. E. Medina-Rivero thanks to CONACyT fellowship No. 157496. We thank Dr. Roderick Mackie (University of Illinois) for English corrections and Dr. A. De Las Pe{\~n}as and Dr. I. Casta{\~n}o (IPICyT) for critical reading of the manuscript.",

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doi = "10.1007/s10529-007-9395-5",

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Medina-Rivero, E, Balderas-Hernández, VE, Ordoñez-Acevedo, LG, Paz-Maldonado, LMT, Barba-De La Rosa, AP & De León-Rodríguez, A 2007, 'Modified penicillin acylase signal peptide allows the periplasmic production of soluble human interferon-γ but not of soluble human interleukin-2 by the Tat pathway in Escherichia coli', Biotechnology Letters, vol. 29, no. 9, pp. 1369-1374. https://doi.org/10.1007/s10529-007-9395-5

Modified penicillin acylase signal peptide allows the periplasmic production of soluble human interferon-γ but not of soluble human interleukin-2 by the Tat pathway in Escherichia coli. / Medina-Rivero, E.; Balderas-Hernández, V. E.; Ordoñez-Acevedo, L. G. et al.
In: Biotechnology Letters, Vol. 29, No. 9, 09.2007, p. 1369-1374.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Modified penicillin acylase signal peptide allows the periplasmic production of soluble human interferon-γ but not of soluble human interleukin-2 by the Tat pathway in Escherichia coli

AU - Medina-Rivero, E.

AU - Balderas-Hernández, V. E.

AU - Ordoñez-Acevedo, L. G.

AU - Paz-Maldonado, L. M.T.

AU - Barba-De La Rosa, A. P.

AU - De León-Rodríguez, A.

N1 - Funding Information: Acknowledgments This work was partially supported by CONACyT Grant J39639-Q and a FOMIX grant FMSLP-2002–4100. E. Medina-Rivero thanks to CONACyT fellowship No. 157496. We thank Dr. Roderick Mackie (University of Illinois) for English corrections and Dr. A. De Las Peñas and Dr. I. Castaño (IPICyT) for critical reading of the manuscript.

PY - 2007/9

Y1 - 2007/9

N2 - Production of periplasmic human interferon-γ (hINF-γ) and human interleukin-2 (hIL-2) by the Tat translocation pathway in Escherichia coli BL21-SI was evaluated. The expression was obtained using the pEMR vector which contains the Tat-dependent modified penicillin acylase signal peptide (mSPpac) driven by the T7 promoter. The mSPpac-hINF-γ was processed and the protein was transported to periplasm. Up to 30.1% of hINF-γ was found in the periplasmic soluble fraction, whereas only 15% of the mSPpac-hIL-2 was processed, but hIL-2 was not found in the periplasmic soluble fraction.

AB - Production of periplasmic human interferon-γ (hINF-γ) and human interleukin-2 (hIL-2) by the Tat translocation pathway in Escherichia coli BL21-SI was evaluated. The expression was obtained using the pEMR vector which contains the Tat-dependent modified penicillin acylase signal peptide (mSPpac) driven by the T7 promoter. The mSPpac-hINF-γ was processed and the protein was transported to periplasm. Up to 30.1% of hINF-γ was found in the periplasmic soluble fraction, whereas only 15% of the mSPpac-hIL-2 was processed, but hIL-2 was not found in the periplasmic soluble fraction.

KW - Periplasm

KW - Signal peptide

KW - Tat pathway

KW - Therapeutic protein

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DO - 10.1007/s10529-007-9395-5

M3 - Artículo

SN - 0141-5492

VL - 29

SP - 1369

EP - 1374

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 9

ER -

Medina-Rivero E, Balderas-Hernández VE, Ordoñez-Acevedo LG, Paz-Maldonado LMT, Barba-De La Rosa AP, De León-Rodríguez A. Modified penicillin acylase signal peptide allows the periplasmic production of soluble human interferon-γ but not of soluble human interleukin-2 by the Tat pathway in Escherichia coli. Biotechnology Letters. 2007 Sep;29(9):1369-1374. doi: 10.1007/s10529-007-9395-5

Modified penicillin acylase signal peptide allows the periplasmic production of soluble human interferon-γ but not of soluble human interleukin-2 by the Tat pathway in Escherichia coli

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Keywords

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