TY - JOUR
T1 - Microbial profile of the leachate from Mexico city's bordo poniente composting plant
T2 - An inoculum to digest organicwaste
AU - Bravo, Aixa Kari Gállego
AU - Serrano, Daniel Alejandro Salcedo
AU - Jiménez, Gloria López
AU - Nirmalkar, Khemlal
AU - Murugesan, Selvasankar
AU - García-Mena, Jaime
AU - Castillo, María Eugenia Gutiérrez
AU - Gálvez, Luis Raúl Tovar
N1 - Publisher Copyright:
© 2019 by the authors.
PY - 2019
Y1 - 2019
N2 - In recent years, municipal solid waste (MSW) management has become a complex problem worldwide. Similarly, Mexico City is facing such a situation for the management and treatment of organic fraction of municipal solid waste (OFMSW). Therefore, in this work, we investigated whether leachate from the composting plant, Bordo Poniente, located in Mexico City can be used as an inoculum for the treatment of OFMSW using thermophilic anaerobic digestion (AD) with a hydraulic retention time of 30 days. We analyzed the physicochemical properties of the leachate and performed a biochemical methane potential test. Archaeal and bacterial diversity was also identified using high throughput DNA sequencing of 16S rDNA libraries. Methane yield was 0.29 m3 CH4/kg VSadded in the positive control and 0.16 m3 CH4/kg VSadded in the treatment group. The phylum, Bacteroidetes, and genus, Methanosarcina, prevailed in the leachate. However, in thermophilic conditions, the microbial communities changed, and the phylum, Firmicutes, genera, Methanoculleus, and candidate genus, vadinCA11, were dominant in the treatment group. We concluded that the leachate contains a suitable initial charge of many active bacteria and methanogenic archaea which contribute to the AD process, hence it can be used as an inoculum for the treatment of OFMSW.
AB - In recent years, municipal solid waste (MSW) management has become a complex problem worldwide. Similarly, Mexico City is facing such a situation for the management and treatment of organic fraction of municipal solid waste (OFMSW). Therefore, in this work, we investigated whether leachate from the composting plant, Bordo Poniente, located in Mexico City can be used as an inoculum for the treatment of OFMSW using thermophilic anaerobic digestion (AD) with a hydraulic retention time of 30 days. We analyzed the physicochemical properties of the leachate and performed a biochemical methane potential test. Archaeal and bacterial diversity was also identified using high throughput DNA sequencing of 16S rDNA libraries. Methane yield was 0.29 m3 CH4/kg VSadded in the positive control and 0.16 m3 CH4/kg VSadded in the treatment group. The phylum, Bacteroidetes, and genus, Methanosarcina, prevailed in the leachate. However, in thermophilic conditions, the microbial communities changed, and the phylum, Firmicutes, genera, Methanoculleus, and candidate genus, vadinCA11, were dominant in the treatment group. We concluded that the leachate contains a suitable initial charge of many active bacteria and methanogenic archaea which contribute to the AD process, hence it can be used as an inoculum for the treatment of OFMSW.
KW - High throughput DNA sequencing
KW - Leachate
KW - Methanogens
KW - Microbial diversity
KW - Organic fraction of municipal solid waste
KW - Thermophilic anaerobic digestion
UR - http://www.scopus.com/inward/record.url?scp=85068412981&partnerID=8YFLogxK
U2 - 10.3390/en12122343
DO - 10.3390/en12122343
M3 - Artículo
AN - SCOPUS:85068412981
SN - 1996-1073
VL - 12
JO - Energies
JF - Energies
IS - 12
M1 - 2343
ER -