Methyl tert-butyl ether biodegradation by microbial consortia obtained from soil samples of gasoline-polluted sites in Mexico

Marcia Morales; Elia Velázquez; Janet Jan; Sergio Revah; Uriel González; Elías Razo-Flores

doi:10.1023/B:BILE.0000015424.04776.55

Methyl tert-butyl ether biodegradation by microbial consortia obtained from soil samples of gasoline-polluted sites in Mexico

Marcia Morales, Elia Velázquez, Janet Jan, Sergio Revah, Uriel González, Elías Razo-Flores

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Microbial consortia obtained from soil samples of gasoline-polluted sites were individually enriched with pentane, hexane, isooctane and toluene. Cometabolism with methyl tert-butyl ether, (MTBE), gave maximum degradation rates of 49, 12, 32 and 0 mg g_protein^-1 h^-1, respectively. MTBE was fully degraded even when pentane was completely depleted with a cometabolic coefficient of 1 mg_MTBE mg_pentane ^-1. The analysis of 16S rDNA from isolated microorganisms in the pentane-adapted consortia showed that microorganisms could be assigned to Pseudomonas. This is the first work reporting the cometabolic mineralization of MTBE by consortium of this genus.

Original language	English
Pages (from-to)	269-275
Number of pages	7
Journal	Biotechnology Letters
Volume	26
Issue number	4
DOIs	https://doi.org/10.1023/B:BILE.0000015424.04776.55
State	Published - Feb 2004
Externally published	Yes

Keywords

Biodegradation
Cometabolism
Consortia
Methyl tert-butyl ether
Oxygenated additives
Pseudomonas

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10.1023/B:BILE.0000015424.04776.55

Cite this

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title = "Methyl tert-butyl ether biodegradation by microbial consortia obtained from soil samples of gasoline-polluted sites in Mexico",

abstract = "Microbial consortia obtained from soil samples of gasoline-polluted sites were individually enriched with pentane, hexane, isooctane and toluene. Cometabolism with methyl tert-butyl ether, (MTBE), gave maximum degradation rates of 49, 12, 32 and 0 mg gprotein-1 h-1, respectively. MTBE was fully degraded even when pentane was completely depleted with a cometabolic coefficient of 1 mgMTBE mgpentane -1. The analysis of 16S rDNA from isolated microorganisms in the pentane-adapted consortia showed that microorganisms could be assigned to Pseudomonas. This is the first work reporting the cometabolic mineralization of MTBE by consortium of this genus.",

keywords = "Biodegradation, Cometabolism, Consortia, Methyl tert-butyl ether, Oxygenated additives, Pseudomonas",

author = "Marcia Morales and Elia Vel{\'a}zquez and Janet Jan and Sergio Revah and Uriel Gonz{\'a}lez and El{\'i}as Razo-Flores",

note = "Funding Information: This work was supported by research grants from Instituto Mexicano del Petr{\'o}leo (D.00888) and CON-ACyT (J33913-B).",

year = "2004",

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doi = "10.1023/B:BILE.0000015424.04776.55",

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volume = "26",

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journal = "Biotechnology Letters",

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T1 - Methyl tert-butyl ether biodegradation by microbial consortia obtained from soil samples of gasoline-polluted sites in Mexico

AU - Morales, Marcia

AU - Velázquez, Elia

AU - Jan, Janet

AU - Revah, Sergio

AU - González, Uriel

AU - Razo-Flores, Elías

N1 - Funding Information: This work was supported by research grants from Instituto Mexicano del Petróleo (D.00888) and CON-ACyT (J33913-B).

PY - 2004/2

Y1 - 2004/2

N2 - Microbial consortia obtained from soil samples of gasoline-polluted sites were individually enriched with pentane, hexane, isooctane and toluene. Cometabolism with methyl tert-butyl ether, (MTBE), gave maximum degradation rates of 49, 12, 32 and 0 mg gprotein-1 h-1, respectively. MTBE was fully degraded even when pentane was completely depleted with a cometabolic coefficient of 1 mgMTBE mgpentane -1. The analysis of 16S rDNA from isolated microorganisms in the pentane-adapted consortia showed that microorganisms could be assigned to Pseudomonas. This is the first work reporting the cometabolic mineralization of MTBE by consortium of this genus.

AB - Microbial consortia obtained from soil samples of gasoline-polluted sites were individually enriched with pentane, hexane, isooctane and toluene. Cometabolism with methyl tert-butyl ether, (MTBE), gave maximum degradation rates of 49, 12, 32 and 0 mg gprotein-1 h-1, respectively. MTBE was fully degraded even when pentane was completely depleted with a cometabolic coefficient of 1 mgMTBE mgpentane -1. The analysis of 16S rDNA from isolated microorganisms in the pentane-adapted consortia showed that microorganisms could be assigned to Pseudomonas. This is the first work reporting the cometabolic mineralization of MTBE by consortium of this genus.

KW - Biodegradation

KW - Cometabolism

KW - Consortia

KW - Methyl tert-butyl ether

KW - Oxygenated additives

KW - Pseudomonas

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U2 - 10.1023/B:BILE.0000015424.04776.55

DO - 10.1023/B:BILE.0000015424.04776.55

M3 - Artículo

SN - 0141-5492

VL - 26

SP - 269

EP - 275

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 4

ER -

Methyl tert-butyl ether biodegradation by microbial consortia obtained from soil samples of gasoline-polluted sites in Mexico

Abstract

Keywords

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