TY - JOUR
T1 - Mechanisms of relaxant action of a crude hexane extract of Gnaphalium liebmannii in guinea pig tracheal smooth muscle
AU - Sánchez-Mendoza, María Elena
AU - Torres, Gabriela
AU - Arrieta, Jesús
AU - Aguilar, Abigail
AU - Castillo-Henkel, Carlos
AU - Navarrete, Andrés
N1 - Funding Information:
This work was suported by grands of Dirección General de Asuntos del Personal Académico DGAPA-UNAM IN201506 and CONACYT (Proyecto C01-018). M.E. Sánchez-Mendoza acknowledges fellowship from CONACyT (176651) and Programa Institucional de Formación de Investigadores (PIFI), IPN, to carry out graduate studies.
PY - 2007/4/20
Y1 - 2007/4/20
N2 - We investigated the mechanisms of action of Gnaphalium liebmannii which is used as a folk medicine in México for treating various respiratory diseases such as gripe, fever, asthma, cough, cold, bronchitis, expectorating, and bronchial affections. The tension changes of guinea pig tracheal segments were isometrically recorder on a polygraph. Hexane extract of Gnaphalium liebmannii was the most active relaxant extract (IC30 = 54.23 ± 19.79 μg/mL with 99.5 ± 3.2 % of relaxation), followed by dichloromethane extract (IC30 = 120.22 ± 5.27 μg/mL) and methanol extract (IC30 = 190.25 ± 30.02 μg/mL). Hexane extract produced a parallel rightward shift of the concentration-response curve of carbachol in a competitive manner (pA2 = -2.4), but did not modify the concentration-response curves for histamine. The relaxant effect of hexane extract of Gnaphalium liebmannii was unaffected by the presence of propranolol (3 × 10-6 M) or glibenclamide (10 μM). However hexane extract produced a leftward shifts of the concentration-response curve of forskolin (10-8 to 10-3 M), nitroprusside (10-10 to 10-6 M), isoproterenol (3 × 10-10 to 3 × 10-5 M) and aminophylline (10-11 to 10-2 M). The above results suggest that Gnaphalium liebmannii induce relaxation of the tracheal muscle, probably via phosphodiesterase inhibition. The bronchodilator effect of Gnaphalium liebmannii might explain in part their traditional use as anti-asthmatic remedy.
AB - We investigated the mechanisms of action of Gnaphalium liebmannii which is used as a folk medicine in México for treating various respiratory diseases such as gripe, fever, asthma, cough, cold, bronchitis, expectorating, and bronchial affections. The tension changes of guinea pig tracheal segments were isometrically recorder on a polygraph. Hexane extract of Gnaphalium liebmannii was the most active relaxant extract (IC30 = 54.23 ± 19.79 μg/mL with 99.5 ± 3.2 % of relaxation), followed by dichloromethane extract (IC30 = 120.22 ± 5.27 μg/mL) and methanol extract (IC30 = 190.25 ± 30.02 μg/mL). Hexane extract produced a parallel rightward shift of the concentration-response curve of carbachol in a competitive manner (pA2 = -2.4), but did not modify the concentration-response curves for histamine. The relaxant effect of hexane extract of Gnaphalium liebmannii was unaffected by the presence of propranolol (3 × 10-6 M) or glibenclamide (10 μM). However hexane extract produced a leftward shifts of the concentration-response curve of forskolin (10-8 to 10-3 M), nitroprusside (10-10 to 10-6 M), isoproterenol (3 × 10-10 to 3 × 10-5 M) and aminophylline (10-11 to 10-2 M). The above results suggest that Gnaphalium liebmannii induce relaxation of the tracheal muscle, probably via phosphodiesterase inhibition. The bronchodilator effect of Gnaphalium liebmannii might explain in part their traditional use as anti-asthmatic remedy.
KW - Asthma
KW - Flavonoids
KW - Gnaphalium liebmannii
KW - Guinea-pig trachea
KW - Mexican traditional medicine
UR - http://www.scopus.com/inward/record.url?scp=34147148447&partnerID=8YFLogxK
U2 - 10.1016/j.jep.2006.11.001
DO - 10.1016/j.jep.2006.11.001
M3 - Artículo
SN - 0378-8741
VL - 111
SP - 142
EP - 147
JO - Journal of Ethnopharmacology
JF - Journal of Ethnopharmacology
IS - 1
ER -