Magnetic paper-based ELISA for IgM-dengue detection

G. A. Ortega; S. Pérez-Rodríguez; E. Reguera

doi:10.1039/c6ra25992h

Magnetic paper-based ELISA for IgM-dengue detection

G. A. Ortega, S. Pérez-Rodríguez, E. Reguera

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

This contribution reports a novel "Magnetic Paper-Based ELISA" using core-shell magnetite@polydopamine nanoparticles supported on a Whatman paper-like new solid immunoassay platform specifically for IgM-dengue antibodies recognition as the proof-of-concept target for antibodies isotype IgM detection. Affordable procedures to deposit magnetite nanoparticles on cellulose paper sheets (Whatman type-1 and ss903) and to conjugate such nanoparticles with anti human-IgM antibodies using polydopamine as linker are reported. Structural features, magnetic behavior, coating homogeneity, and the nanoparticles/linked antibodies ratio were determined. Additionally, "Magnetic Paper-Based ELISA" for IgM-dengue antibodies detection provides a system with improved analytical response (two orders more sensitive with a 700 times lower limit of detection (LOD) than traditional ELISA or using magnetic beads without depositing), appropriate accuracy for real sample detection, low cost, easy manufacturing, and effortless and easy handling.

Original language	English
Pages (from-to)	4921-4932
Number of pages	12
Journal	RSC Advances
Volume	7
Issue number	9
DOIs	https://doi.org/10.1039/c6ra25992h
State	Published - 2017
Externally published	Yes

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title = "Magnetic paper-based ELISA for IgM-dengue detection",

abstract = "This contribution reports a novel {"}Magnetic Paper-Based ELISA{"} using core-shell magnetite@polydopamine nanoparticles supported on a Whatman paper-like new solid immunoassay platform specifically for IgM-dengue antibodies recognition as the proof-of-concept target for antibodies isotype IgM detection. Affordable procedures to deposit magnetite nanoparticles on cellulose paper sheets (Whatman type-1 and ss903) and to conjugate such nanoparticles with anti human-IgM antibodies using polydopamine as linker are reported. Structural features, magnetic behavior, coating homogeneity, and the nanoparticles/linked antibodies ratio were determined. Additionally, {"}Magnetic Paper-Based ELISA{"} for IgM-dengue antibodies detection provides a system with improved analytical response (two orders more sensitive with a 700 times lower limit of detection (LOD) than traditional ELISA or using magnetic beads without depositing), appropriate accuracy for real sample detection, low cost, easy manufacturing, and effortless and easy handling.",

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AU - Ortega, G. A.

AU - Pérez-Rodríguez, S.

AU - Reguera, E.

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AB - This contribution reports a novel "Magnetic Paper-Based ELISA" using core-shell magnetite@polydopamine nanoparticles supported on a Whatman paper-like new solid immunoassay platform specifically for IgM-dengue antibodies recognition as the proof-of-concept target for antibodies isotype IgM detection. Affordable procedures to deposit magnetite nanoparticles on cellulose paper sheets (Whatman type-1 and ss903) and to conjugate such nanoparticles with anti human-IgM antibodies using polydopamine as linker are reported. Structural features, magnetic behavior, coating homogeneity, and the nanoparticles/linked antibodies ratio were determined. Additionally, "Magnetic Paper-Based ELISA" for IgM-dengue antibodies detection provides a system with improved analytical response (two orders more sensitive with a 700 times lower limit of detection (LOD) than traditional ELISA or using magnetic beads without depositing), appropriate accuracy for real sample detection, low cost, easy manufacturing, and effortless and easy handling.

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JO - RSC Advances

JF - RSC Advances

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Magnetic paper-based ELISA for IgM-dengue detection

Abstract

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