Low cost and optical detection from fluorescence of Ochratoxin A

D. Bueno, R. Munoz, J. L. Marty

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

Ochratoxin A (OTA) is one of the most abundant food-contaminating mycotoxins, is detected worldwide in various food and feed sources and its toxicity in animals is more potent than other metabolites. Detected by the analytical methods, in spite of their high sensitivity, these methods are not suitable for analysis in-situ. Hence, this work consists to develop a low cost and portable system to quantify the concentrations of OTA through ownership of fluorescence. That occurs when it is excited with ultraviolet light (UV) at 375nm. First, an UV emitter and a silicon photodiodes were used; the limit of detection (LOD) was 250 ng/mL, a battery as source and an acquisition system with LabVIEW to process the information in a computer. To detect lower levels, a camera and red, blue and green (RGB) components were employed. The LOD was 5 μg/L. The camera detected the fluorescence in the sample when this was excited with UV light, the variation in the intensity of the color is measured and it has relation with the concentration of the sample.

Original languageEnglish
Title of host publication2017 Global Medical Engineering Physics Exchanges/Pan American Health Care Exchanges, GMEPE/PAHCE 2017
PublisherIEEE Computer Society
ISBN (Electronic)9781538615201
DOIs
StatePublished - 7 Jul 2017
Externally publishedYes
Event2017 Global Medical Engineering Physics Exchanges/Pan American Health Care Exchanges, GMEPE/PAHCE 2017 - Tuxtla-Gutierrez, Mexico
Duration: 20 Mar 201725 Mar 2017

Publication series

NamePan American Health Care Exchanges, PAHCE
Volume2017-March
ISSN (Print)2327-8161
ISSN (Electronic)2327-817X

Conference

Conference2017 Global Medical Engineering Physics Exchanges/Pan American Health Care Exchanges, GMEPE/PAHCE 2017
Country/TerritoryMexico
CityTuxtla-Gutierrez
Period20/03/1725/03/17

Keywords

  • Fluorescence
  • OTA
  • Optic methods

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