TY - JOUR
T1 - Linezolid diminishes inflammatory cytokine production from human peripheral blood mononuclear cells
AU - Garcia-Roca, Pilar
AU - Mancilla-Ramirez, Javier
AU - Santos-Segura, Araceli
AU - Fernández-Avilés, Marisol
AU - Calderon-Jaimes, Ernesto
N1 - Funding Information:
There was no funding from the pharmaceutical company for this study. The researchers did not receive any financial benefits. The study was supported by Children's Hospital of Mexico Federico Gómez.
PY - 2006/1
Y1 - 2006/1
N2 - Background. Active peptides produced by monocytes, in response to endotoxin, initiate and maintain the acute phase of inflammatory response. Some antibiotics have been reported to have immunomodulatory effects in addition to their antimicrobial activity. We examined the effect of linezolid on cytokine synthesis. Methods. The modulatory effects of erythromycin and linezolid were evaluated in LPS-stimulated human peripheral blood mononuclear cells (PBMCs). Blood was obtained by venipuncture from healthy donor volunteers. PBMCs were separated by Ficoll-Paque. More than 90% of the cells were monocytes as determined by esterase staining. Cells were incubated in the presence of LPS, with or without various concentrations of erythromycin and linezolid. The concentration of each cytokine was determined by ELISA with commercially available reagents. Results. The two drugs suppressed significantly the synthesis of the cytokines tested in a concentration-dependent manner. Conclusions. These data indicate that antibacterial agents may modify acute phase inflammatory response through their effects on cytokines synthesis by monocytes.
AB - Background. Active peptides produced by monocytes, in response to endotoxin, initiate and maintain the acute phase of inflammatory response. Some antibiotics have been reported to have immunomodulatory effects in addition to their antimicrobial activity. We examined the effect of linezolid on cytokine synthesis. Methods. The modulatory effects of erythromycin and linezolid were evaluated in LPS-stimulated human peripheral blood mononuclear cells (PBMCs). Blood was obtained by venipuncture from healthy donor volunteers. PBMCs were separated by Ficoll-Paque. More than 90% of the cells were monocytes as determined by esterase staining. Cells were incubated in the presence of LPS, with or without various concentrations of erythromycin and linezolid. The concentration of each cytokine was determined by ELISA with commercially available reagents. Results. The two drugs suppressed significantly the synthesis of the cytokines tested in a concentration-dependent manner. Conclusions. These data indicate that antibacterial agents may modify acute phase inflammatory response through their effects on cytokines synthesis by monocytes.
KW - Effect of antibiotics on cytokine production
KW - Modulatory effects of antibiotics
KW - Production of cytokines
UR - http://www.scopus.com/inward/record.url?scp=28444457464&partnerID=8YFLogxK
U2 - 10.1016/j.arcmed.2005.05.022
DO - 10.1016/j.arcmed.2005.05.022
M3 - Artículo
C2 - 16314183
SN - 0188-4409
VL - 37
SP - 31
EP - 35
JO - Archives of Medical Research
JF - Archives of Medical Research
IS - 1
ER -