TY - JOUR
T1 - Lactic acid bacteria production from whey
AU - Mondragón-Parada, María Elena
AU - Nájera-Martínez, Minerva
AU - Juárez-Ramírez, Cleotilde
AU - Galíndez-Mayer, Juvencio
AU - Ruiz-Ordaz, Nora
AU - Cristiani-Urbina, Eliseo
N1 - Funding Information:
This work was also supported by a grant from the Comisión de Operación y Fomento de Actividades Académicas, Instituto Politécnico Nacional, México.
Funding Information:
We gratefully acknowledge financial support provided by the Secretaría de Investigación y Posgrado, I.P.N. México.
PY - 2006/9
Y1 - 2006/9
N2 - The main purpose of this work was to isolate and characterize lactic acid bacteria (LAB) strains to be used for biomass production using a whey-based medium supplemented with an ammonium salt and with very low levels of yeast extract (0.25 g/L). Five strains of LAB were isolated from naturally soured milk after enrichment in whey-based medium. One bacterial isolate, designated MNM2, exhibited a remarkable capability to utilize whey lactose and give a high biomass yield on lactose. This strain was identified as Lactobacillus casei by its 16S rDNA sequence. A kinetic study of cell growth, lactose consumption, and titratable acidity production of this bacterial strain was performed in a bioreactor. The biomass yield on lactose, the percentage of lactose consumption, and the maximum increase in cell mass obtained in the bioreactor were 0.165 g of biomass/g of lactose, 100%, and 2.0 g/L, respectively, which were 1.44, 1.11, and 2.35 times higher than those found in flask cultures. The results suggest that it is possible to produce LAB biomass from a whey-based medium supplemented with minimal amounts of yeast extract.
AB - The main purpose of this work was to isolate and characterize lactic acid bacteria (LAB) strains to be used for biomass production using a whey-based medium supplemented with an ammonium salt and with very low levels of yeast extract (0.25 g/L). Five strains of LAB were isolated from naturally soured milk after enrichment in whey-based medium. One bacterial isolate, designated MNM2, exhibited a remarkable capability to utilize whey lactose and give a high biomass yield on lactose. This strain was identified as Lactobacillus casei by its 16S rDNA sequence. A kinetic study of cell growth, lactose consumption, and titratable acidity production of this bacterial strain was performed in a bioreactor. The biomass yield on lactose, the percentage of lactose consumption, and the maximum increase in cell mass obtained in the bioreactor were 0.165 g of biomass/g of lactose, 100%, and 2.0 g/L, respectively, which were 1.44, 1.11, and 2.35 times higher than those found in flask cultures. The results suggest that it is possible to produce LAB biomass from a whey-based medium supplemented with minimal amounts of yeast extract.
KW - Ammonium salts
KW - Biomass
KW - Lactic acid bacteria
KW - Whey
KW - Yeast extract
UR - http://www.scopus.com/inward/record.url?scp=33748620280&partnerID=8YFLogxK
U2 - 10.1385/ABAB:134:3:223
DO - 10.1385/ABAB:134:3:223
M3 - Artículo
SN - 0273-2289
VL - 134
SP - 223
EP - 232
JO - Applied Biochemistry and Biotechnology
JF - Applied Biochemistry and Biotechnology
IS - 3
ER -