TY - JOUR
T1 - Kocuria arsenatis sp. Nov., An arsenic-resistant endophytic actinobacterium associated with Prosopis laegivata grown on high-arsenic-polluted mine tailing
AU - Román-Ponce, Brenda
AU - Wang, Dan
AU - Soledad Vásquez-Murrieta, María
AU - Feng Chen, Wen
AU - los Santos, Paulina Estrada De
AU - Hua Sui, Xin
AU - Tao Wang, En
N1 - Publisher Copyright:
© 2015 IUMS.
PY - 2016/2
Y1 - 2016/2
N2 - A Gram-stain-positive, aerobic, non-motile, coccoid, arsenic-resistant actinobacterial strain, designated CM1E1T, was isolated from the lateral root tissue of Prosopis laegivata grown on a mine tailing in San Luis Potosi, Mexico. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CM1E1T was clustered closely with species of the genus Kocuria, and showed the highest sequence similarity of 98.7 % to Kocuria rhizophila TA68T. The DNA G+C content of strain CM1E1T was 74.5 mol% (Tm). The major fatty acids were anteiso-C15: 0, anteiso-C17: 0 and iso-C15: 0. The peptidoglycan of the cell wall contained lysine and alanine. The major respiratory quinones were MK-7(H2) and MK-8(H2). On the basis of the phenotypic characterization, phylogenetic relationships and chemotaxonomic analyses, strain CM1E1T represents a novel species of the genus Kocuria, for which the name Kocuria arsenatis sp. nov. is proposed. The type strain is CM1E1T (=CCBAU 101092T=HAMBI 3625T=LMG 28671T).
AB - A Gram-stain-positive, aerobic, non-motile, coccoid, arsenic-resistant actinobacterial strain, designated CM1E1T, was isolated from the lateral root tissue of Prosopis laegivata grown on a mine tailing in San Luis Potosi, Mexico. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CM1E1T was clustered closely with species of the genus Kocuria, and showed the highest sequence similarity of 98.7 % to Kocuria rhizophila TA68T. The DNA G+C content of strain CM1E1T was 74.5 mol% (Tm). The major fatty acids were anteiso-C15: 0, anteiso-C17: 0 and iso-C15: 0. The peptidoglycan of the cell wall contained lysine and alanine. The major respiratory quinones were MK-7(H2) and MK-8(H2). On the basis of the phenotypic characterization, phylogenetic relationships and chemotaxonomic analyses, strain CM1E1T represents a novel species of the genus Kocuria, for which the name Kocuria arsenatis sp. nov. is proposed. The type strain is CM1E1T (=CCBAU 101092T=HAMBI 3625T=LMG 28671T).
UR - http://www.scopus.com/inward/record.url?scp=84960332743&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.000830
DO - 10.1099/ijsem.0.000830
M3 - Artículo
AN - SCOPUS:84960332743
SN - 1466-5026
VL - 66
SP - 1027
EP - 1033
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 2
M1 - 000830
ER -