TY - JOUR
T1 - Kluyveromyces marxianus CDBB-L-278
T2 - A wild inulinase hyperproducing strain
AU - Cruz-Guerrero, Alma
AU - Garcia-Peña, Ines
AU - Barzana, Eduardo
AU - Garcia-Garibay, Mariano
AU - Gomez-Ruiz, Lorena
N1 - Funding Information:
This project was financially supported by the International Foundation for Science (Sweden). The authors wish to thank Miss Helen L. Rawson for reading the manuscript.
PY - 1995
Y1 - 1995
N2 - Kluyveromyces marxianus CDBB-L-278 is an inulinase hyperproducing strain. It was able to grow in a medium containing inulin as the unique carbon source in the presence of 2-deoxyglucose. It produced up to 3.3 times the activity of the control strain K. marxianus NCYC-1429 in an inulin medium, and 3.6 times in a medium with glycerol as the sole carbon source. Although the strain CDBB-L-278 was able to produce inulinase in the presence of 2-deoxyglucose, it was demonstrated that it is not a de-repressed strain since enzyme production was reduced when the concentration of glucose or fructose was increased in the medium. Since inulinase was produced in a glycerol medium without an inducer, it can be considered that the enzyme production was partially constitutive in K. marxianus CDBB-L-278 as well as strain NCYC-1429. The inulinase from K. marxianus CDBB-L-278 was characterized. It had a higher affinity for inulin than for sucrose. Temperature and pH profiles were different for both of these two substrates. The enzyme was stable to high temperatures, with a half-life of 180 min at 50°C.
AB - Kluyveromyces marxianus CDBB-L-278 is an inulinase hyperproducing strain. It was able to grow in a medium containing inulin as the unique carbon source in the presence of 2-deoxyglucose. It produced up to 3.3 times the activity of the control strain K. marxianus NCYC-1429 in an inulin medium, and 3.6 times in a medium with glycerol as the sole carbon source. Although the strain CDBB-L-278 was able to produce inulinase in the presence of 2-deoxyglucose, it was demonstrated that it is not a de-repressed strain since enzyme production was reduced when the concentration of glucose or fructose was increased in the medium. Since inulinase was produced in a glycerol medium without an inducer, it can be considered that the enzyme production was partially constitutive in K. marxianus CDBB-L-278 as well as strain NCYC-1429. The inulinase from K. marxianus CDBB-L-278 was characterized. It had a higher affinity for inulin than for sucrose. Temperature and pH profiles were different for both of these two substrates. The enzyme was stable to high temperatures, with a half-life of 180 min at 50°C.
KW - Kluyveromyces marxianus
KW - catabolite repression
KW - inulinase
KW - β-fructofuranosidase
UR - http://www.scopus.com/inward/record.url?scp=0029154376&partnerID=8YFLogxK
U2 - 10.1016/0922-338X(95)93212-3
DO - 10.1016/0922-338X(95)93212-3
M3 - Artículo
SN - 0922-338X
VL - 80
SP - 159
EP - 163
JO - Journal of Fermentation and Bioengineering
JF - Journal of Fermentation and Bioengineering
IS - 2
ER -