TY - JOUR
T1 - Kinetics of eosinophils during development of the cellular infiltrate surrounding the nurse cell of trichinella spiralis in experimentally infected mice
AU - Vega-Sánchez, Vicente
AU - Gómez-De-anda, Fabián Ricardo
AU - Calderón-Domínguez, Georgina
AU - Ramírez-Y-ramírez, Mary Carmen Del Sol
AU - Reyes-Rodríguez, Nydia E.
AU - Zepeda-Velázquez, Andrea P.
AU - Tapia-Romero, Raquel
AU - De-La-rosa-arana, Jorge Luis
N1 - Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/11
Y1 - 2021/11
N2 - We study the kinetics of eosinophils during the development of the cellular infiltrate surrounding the nurse cell of Trichinella spiralis (T. spiralis) in experimentally infected mice. Male CD1 mice were experimentally infected with 50 viable muscle larvae of the MSUS/MEX/91/CM-91 T. spiralis strain. Tongues and diaphragms were obtained daily from days 13 to 39 post infection. Diaphragms were compressed and subjected to Giemsa stain. Tongues were histologically sectioned and stained with erythrosine B or hematoxylin and eosin. The cellular infiltrate and the nurse cell-larva complex were detected by optical microscopy since day 16 post infection. The size of the larva increased exponentially during the course of the infection. The kinetics of eosinophils showed a multimodal trend, with a bimodal predominance. The maximum peaks were reached on days 21 and 27 post infection. The results of this study demonstrate that eosinophils occur abundantly in two transcendent moments of the T. spiralis life cycle: first, when the stage 1 larva invades the myocyte and second when the nurse cell-larva complex has been fully developed. These results help one to understand the immunobiology of T. spiralis, highlighting the importance of eosinophils in the survival of the larva in skeletal muscle. Further studies are needed to characterize the cell populations that comprise the cellular infiltrate during the development of the mother cell.
AB - We study the kinetics of eosinophils during the development of the cellular infiltrate surrounding the nurse cell of Trichinella spiralis (T. spiralis) in experimentally infected mice. Male CD1 mice were experimentally infected with 50 viable muscle larvae of the MSUS/MEX/91/CM-91 T. spiralis strain. Tongues and diaphragms were obtained daily from days 13 to 39 post infection. Diaphragms were compressed and subjected to Giemsa stain. Tongues were histologically sectioned and stained with erythrosine B or hematoxylin and eosin. The cellular infiltrate and the nurse cell-larva complex were detected by optical microscopy since day 16 post infection. The size of the larva increased exponentially during the course of the infection. The kinetics of eosinophils showed a multimodal trend, with a bimodal predominance. The maximum peaks were reached on days 21 and 27 post infection. The results of this study demonstrate that eosinophils occur abundantly in two transcendent moments of the T. spiralis life cycle: first, when the stage 1 larva invades the myocyte and second when the nurse cell-larva complex has been fully developed. These results help one to understand the immunobiology of T. spiralis, highlighting the importance of eosinophils in the survival of the larva in skeletal muscle. Further studies are needed to characterize the cell populations that comprise the cellular infiltrate during the development of the mother cell.
KW - Cellular infiltrate
KW - Eosinophil
KW - Experimental infection
KW - Nurse cell
KW - Trichinella spiralis
UR - http://www.scopus.com/inward/record.url?scp=85118219737&partnerID=8YFLogxK
U2 - 10.3390/pathogens10111382
DO - 10.3390/pathogens10111382
M3 - Artículo
C2 - 34832538
AN - SCOPUS:85118219737
SN - 2076-0817
VL - 10
JO - Pathogens
JF - Pathogens
IS - 11
M1 - 1382
ER -