TY - JOUR
T1 - Kinetic study of phenol hydroxylase and catechol 1,2-dioxygenase biosynthesis by Candida tropicalis cells grown on different phenolic substrates
AU - Ahuatzi-Chacón, Deifilia
AU - Ordorica-Morales, Guadalupe
AU - Ruiz-Ordaz, Nora
AU - Cristiani-Urbina, Eliseo
AU - Juárez-Ramírez, Cleotilde
AU - Galíndez-Mayer, Juvencio
N1 - Funding Information:
C.J.-R., N.R.-O., E.C.-U. and J.G.-M. are fellow holders of a grant from the Comisión de Operación y Fomento de Actividades Académicas, Instituto Politéc-nico Nacional, Mexico City, Mexico.
PY - 2004/10
Y1 - 2004/10
N2 - When Candida tropicalis was grown on phenol, catechol or resorcinol, the highest levels of specific activity of phenol hydroxylase (EC. 1.14.13.7) and catechol 1,2-dioxygenase (EC. 1.13.11.1) were attained with phenol. With the three aromatic compounds tested, the yeast cells exhibited sharp peaks of specific activity of both enzymes at particular incubation times. Phenol-induced cells containing high levels of both enzymes were capable of degrading rapidly and without delay 4-chlorophenol and 2,6-dichlorophenol, and to a lesser extend pentachlorophenol. However, the yeast could not grow on chlorophenols as major carbon and energy source.
AB - When Candida tropicalis was grown on phenol, catechol or resorcinol, the highest levels of specific activity of phenol hydroxylase (EC. 1.14.13.7) and catechol 1,2-dioxygenase (EC. 1.13.11.1) were attained with phenol. With the three aromatic compounds tested, the yeast cells exhibited sharp peaks of specific activity of both enzymes at particular incubation times. Phenol-induced cells containing high levels of both enzymes were capable of degrading rapidly and without delay 4-chlorophenol and 2,6-dichlorophenol, and to a lesser extend pentachlorophenol. However, the yeast could not grow on chlorophenols as major carbon and energy source.
KW - Candida tropicalis
KW - Catechol 1,2-dioxygenase
KW - Chloroaromatics
KW - Co-metabolism
KW - Phenol hydroxylase
KW - Phenolic compounds
UR - http://www.scopus.com/inward/record.url?scp=11844301642&partnerID=8YFLogxK
U2 - 10.1007/s11274-004-2622-5
DO - 10.1007/s11274-004-2622-5
M3 - Artículo
SN - 0959-3993
VL - 20
SP - 695
EP - 702
JO - World Journal of Microbiology and Biotechnology
JF - World Journal of Microbiology and Biotechnology
IS - 7
ER -