TY - JOUR
T1 - Isolation and purification of two immunodominant antigens from Nocardia brasiliensis
AU - Vera-Cabrera, L.
AU - Salinas-Carmona, M. C.
AU - Welsh, O.
AU - Rodriguez, M. A.
PY - 1992
Y1 - 1992
N2 - Two immunogenic proteins from a crude extract of Nocardia brasiliensis were purified to homogeneity. A 61-kDa protein (P61) was isolated from a 50% ammonium sulfate precipitate in two steps. Initially, P61 was obtained by electroelution in a 10% nondenatured preparative polyacrylamide gel electrophoresis (PAGE). In a second step, the eluate from the nondenatured gel was run in a 12% sodium dodecyl sulfate (SDS) preparative polyacrylamide gel. After elution, a single band was demonstrated by SDS-PAGE and Western blot (immunoblot). Also, a 24-kDa immunogenic protein (P24) was isolated by gel filtration in a Sephadex G-100 column and then by electroelution in a 12% nondenatured polyacrylamide gel. In a previous paper, we showed by Western blot assays that these proteins are recognized by the sera of mycetoma patients and not by sera from mycobacterial-infected or healthy individuals. We consider these proteins to be good candidates for the study of the host- parasite relationship in nocardial infections. The possible clinical application of these purified antigens in a serological diagnosis is discussed.
AB - Two immunogenic proteins from a crude extract of Nocardia brasiliensis were purified to homogeneity. A 61-kDa protein (P61) was isolated from a 50% ammonium sulfate precipitate in two steps. Initially, P61 was obtained by electroelution in a 10% nondenatured preparative polyacrylamide gel electrophoresis (PAGE). In a second step, the eluate from the nondenatured gel was run in a 12% sodium dodecyl sulfate (SDS) preparative polyacrylamide gel. After elution, a single band was demonstrated by SDS-PAGE and Western blot (immunoblot). Also, a 24-kDa immunogenic protein (P24) was isolated by gel filtration in a Sephadex G-100 column and then by electroelution in a 12% nondenatured polyacrylamide gel. In a previous paper, we showed by Western blot assays that these proteins are recognized by the sera of mycetoma patients and not by sera from mycobacterial-infected or healthy individuals. We consider these proteins to be good candidates for the study of the host- parasite relationship in nocardial infections. The possible clinical application of these purified antigens in a serological diagnosis is discussed.
UR - http://www.scopus.com/inward/record.url?scp=0026527973&partnerID=8YFLogxK
U2 - 10.1128/jcm.30.5.1183-1188.1992
DO - 10.1128/jcm.30.5.1183-1188.1992
M3 - Artículo
SN - 0095-1137
VL - 30
SP - 1183
EP - 1188
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 5
ER -