TY - JOUR
T1 - Isolation and molecular characterization of MYB60 in Solanum lycopersicum
AU - Rodríguez-Hoces de la Guardia, Amparo
AU - Ugalde, María Beatriz
AU - Lobos-Diaz, Valeria
AU - Romero-Romero, Jesús Lucina
AU - Meyer-Regueiro, Carlos
AU - Inostroza-Blancheteau, Claudio
AU - Reyes-Diaz, Marjorie
AU - Aquea, Felipe
AU - Arce-Johnson, Patricio
N1 - Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer Nature B.V. part of Springer Nature.
PY - 2021/2
Y1 - 2021/2
N2 - Stomatal closure is a common adaptation response of plants to the onset of drought condition and its regulation is controlled by transcription factors. MYB60, a transcription factor involved in the regulation of light-induced stomatal opening, has been characterized in arabidopsis and grapevine. In this work, we studied the role of MYB60 homolog SIMYB60 in tomato plants. We identified, isolated, and sequenced the SIMYB60 coding sequence, and found domains and motifs characteristic of other MYB60 proteins. We determined that SlMYB60 is mainly expressed in leaves, and its expression is repressed by abscisic acid. Next, we isolated a putative promoter region containing regulatory elements responsible for guard cell expression and other putative regulatory elements related to ABA repression and vascular tissue expression. Protein localization assays demonstrated that SlMYB60 localizes to the nucleus. Finally, SlMYB60 is able to complement the mutant phenotype of atmyb60-1 in Arabidopsis. Together, these results indicate that SlMYB60 is the homologous gene in tomato and potentially offer a molecular target to improve crops.
AB - Stomatal closure is a common adaptation response of plants to the onset of drought condition and its regulation is controlled by transcription factors. MYB60, a transcription factor involved in the regulation of light-induced stomatal opening, has been characterized in arabidopsis and grapevine. In this work, we studied the role of MYB60 homolog SIMYB60 in tomato plants. We identified, isolated, and sequenced the SIMYB60 coding sequence, and found domains and motifs characteristic of other MYB60 proteins. We determined that SlMYB60 is mainly expressed in leaves, and its expression is repressed by abscisic acid. Next, we isolated a putative promoter region containing regulatory elements responsible for guard cell expression and other putative regulatory elements related to ABA repression and vascular tissue expression. Protein localization assays demonstrated that SlMYB60 localizes to the nucleus. Finally, SlMYB60 is able to complement the mutant phenotype of atmyb60-1 in Arabidopsis. Together, these results indicate that SlMYB60 is the homologous gene in tomato and potentially offer a molecular target to improve crops.
KW - Abiotic stress
KW - Drought
KW - MYB60
KW - Stomatal aperture
KW - Tomato
UR - http://www.scopus.com/inward/record.url?scp=85099760803&partnerID=8YFLogxK
U2 - 10.1007/s11033-021-06168-5
DO - 10.1007/s11033-021-06168-5
M3 - Artículo
C2 - 33502700
AN - SCOPUS:85099760803
SN - 0301-4851
VL - 48
SP - 1579
EP - 1587
JO - Molecular Biology Reports
JF - Molecular Biology Reports
IS - 2
ER -