TY - JOUR
T1 - Isolation and identification of naegleria species in irrigation channels for recreational use in mexicali valley, mexico
AU - Bonilla-Lemus, Patricia
AU - Rojas-Hernández, Saúl
AU - Ramírez-Flores, Elizabeth
AU - Castillo-Ramírez, Diego A.
AU - Monsalvo-Reyes, Alejandro Cruz
AU - Ramírez-Flores, Miguel A.
AU - Barrón-Graciano, Karla
AU - Reyes-Batlle, María
AU - Lorenzo-Morales, Jacob
AU - Carrasco-Yépez, María Maricela
N1 - Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2020/10
Y1 - 2020/10
N2 - Members of the genus Naegleria are free-living amoebae that are widely distributed in water and soil environments. Moreover, Naegleria fowleri is a pathogenic amoeba species that causes a fatal disease in the central nervous system known as primary amoebic meningoencephalitis (PAM) in humans. Since most reported infections due to N. fowleri are reported in recreational waters worldwide, this study was aimed to describe the presence of these amoebic genus in Mexicali Valley irrigation channels of recreational use. A total of nine water samples were collected and processed by triplicate, in nine different sites of the Valley. After filtering and culturing the samples, plates were examined, and the observed amoebae were morphologically identified at the genus level. In addition, the pathogenicity of these amoebic isolates was checked, and molecular characterization was performed by PCR/sequencing. The results revealed the presence of Naegleria spp. in all the channels sampled. Finally, molecular identification confirmed the presence of five different species of Naegleria: N. fowleri, N. australiensis, N. gruberi, N. clarki and N. pagei. The presence of these protists, particularly N. fowleri, should be considered as a potential human health risk in the region.
AB - Members of the genus Naegleria are free-living amoebae that are widely distributed in water and soil environments. Moreover, Naegleria fowleri is a pathogenic amoeba species that causes a fatal disease in the central nervous system known as primary amoebic meningoencephalitis (PAM) in humans. Since most reported infections due to N. fowleri are reported in recreational waters worldwide, this study was aimed to describe the presence of these amoebic genus in Mexicali Valley irrigation channels of recreational use. A total of nine water samples were collected and processed by triplicate, in nine different sites of the Valley. After filtering and culturing the samples, plates were examined, and the observed amoebae were morphologically identified at the genus level. In addition, the pathogenicity of these amoebic isolates was checked, and molecular characterization was performed by PCR/sequencing. The results revealed the presence of Naegleria spp. in all the channels sampled. Finally, molecular identification confirmed the presence of five different species of Naegleria: N. fowleri, N. australiensis, N. gruberi, N. clarki and N. pagei. The presence of these protists, particularly N. fowleri, should be considered as a potential human health risk in the region.
KW - Identification
KW - Irrigation channels
KW - Mexicali Valley
KW - Naegleria fowleri
KW - Naegleria spp
UR - http://www.scopus.com/inward/record.url?scp=85092422584&partnerID=8YFLogxK
U2 - 10.3390/pathogens9100820
DO - 10.3390/pathogens9100820
M3 - Artículo
AN - SCOPUS:85092422584
SN - 2076-0817
VL - 9
SP - 1
EP - 14
JO - Pathogens
JF - Pathogens
IS - 10
M1 - 820
ER -