Isolation and cross-amplification of microsatellites in pink abalone (Haliotis corrugata)

N. Díaz-Viloria; R. Pérez-Enríquez; G. Fiore-Amaral; R. S. Burton; P. Cruz

doi:10.1111/j.1471-8286.2007.02054.x

Isolation and cross-amplification of microsatellites in pink abalone (Haliotis corrugata)

N. Díaz-Viloria, R. Pérez-Enríquez, G. Fiore-Amaral, R. S. Burton, P. Cruz

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Ten novel microsatellite loci were isolated in pink abalone, Haliotis corrugata, using (GT)₁₅ and (CT)₁₅enriched genomic libraries. Two previously reported Haliotis kamtschatkana microsatellites cross-amplified in H. corrugata. A set of 12 polymorphic microsatellites were evaluated in a wild population sample (N = 49). The number of alleles ranged from two to 55, and the observed and expected heterozygosities ranged from 0.104 to 0.939 and from 0.213 to 0.982, respectively. Significant deviations from Hardy-Weinberg equilibrium at three loci and no linkage disequilibrium were observed. Haliotis corrugata microsatellites cross-amplified in other abalone species, two in H. fulgens, and seven in H. rufescens.

Original language	English
Pages (from-to)	701-703
Number of pages	3
Journal	Molecular Ecology Resources
Volume	8
Issue number	3
DOIs	https://doi.org/10.1111/j.1471-8286.2007.02054.x
State	Published - May 2008
Externally published	Yes

Keywords

Abalone
Haliotis corrugata
Microsatellite
Pedigree tracing
Population genetics

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10.1111/j.1471-8286.2007.02054.x

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abstract = "Ten novel microsatellite loci were isolated in pink abalone, Haliotis corrugata, using (GT)15 and (CT)15 enriched genomic libraries. Two previously reported Haliotis kamtschatkana microsatellites cross-amplified in H. corrugata. A set of 12 polymorphic microsatellites were evaluated in a wild population sample (N = 49). The number of alleles ranged from two to 55, and the observed and expected heterozygosities ranged from 0.104 to 0.939 and from 0.213 to 0.982, respectively. Significant deviations from Hardy-Weinberg equilibrium at three loci and no linkage disequilibrium were observed. Haliotis corrugata microsatellites cross-amplified in other abalone species, two in H. fulgens, and seven in H. rufescens.",

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T1 - Isolation and cross-amplification of microsatellites in pink abalone (Haliotis corrugata)

AU - Díaz-Viloria, N.

AU - Pérez-Enríquez, R.

AU - Fiore-Amaral, G.

AU - Burton, R. S.

AU - Cruz, P.

PY - 2008/5

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AB - Ten novel microsatellite loci were isolated in pink abalone, Haliotis corrugata, using (GT)15 and (CT)15 enriched genomic libraries. Two previously reported Haliotis kamtschatkana microsatellites cross-amplified in H. corrugata. A set of 12 polymorphic microsatellites were evaluated in a wild population sample (N = 49). The number of alleles ranged from two to 55, and the observed and expected heterozygosities ranged from 0.104 to 0.939 and from 0.213 to 0.982, respectively. Significant deviations from Hardy-Weinberg equilibrium at three loci and no linkage disequilibrium were observed. Haliotis corrugata microsatellites cross-amplified in other abalone species, two in H. fulgens, and seven in H. rufescens.

KW - Abalone

KW - Haliotis corrugata

KW - Microsatellite

KW - Pedigree tracing

KW - Population genetics

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ER -

Isolation and cross-amplification of microsatellites in pink abalone (Haliotis corrugata)

Abstract

Keywords

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