TY - JOUR
T1 - |Isolation and characterization of novel bacteriophages as a potential therapeutic option for Escherichia coli urinary tract infections
AU - González-Villalobos, Edgar
AU - Ribas-Aparicio, Rosa María
AU - Montealegre, Gerardo Erbey Rodea
AU - Belmont-Monroy, Laura
AU - Ortega-García, Yerisaidy
AU - Aparicio-Ozores, Gerardo
AU - Balcázar, José Luis
AU - Eslava-Campos, Carlos Alberto
AU - Hernández-Chiñas, Ulises
AU - Molina-López, José
N1 - Publisher Copyright:
© 2021, The Author(s).
PY - 2021/7
Y1 - 2021/7
N2 - Abstract: Urinary tract infections (UTIs) are mainly caused by uropathogenic Escherichia coli (UPEC), whose impact can be exacerbated by multidrug-resistant (MDR) strains. Effective control strategies are, therefore, urgently needed. Among them, phage therapy represents a suitable alternative. Here, we describe the isolation and characterization of novel phages from wastewater samples, as well as their lytic activity against biofilm and adherence of UPEC to HEp-2 cells. The results demonstrated that phage vB_EcoM-phiEc1 (ϕEc1) belongs to Myoviridae family, whereas vB_EcoS-phiEc3 (ϕEc3) and vB_EcoS-phiEc4 (ϕEc4) belong to Siphoviridae family. Phages showed lytic activity against UPEC and gut commensal strains. Phage ϕEc1 lysed UPEC serogroups, whereas phages ϕEc3 and ϕEc4 lysed only UTI strains with higher prevalence toward the O25 serogroup. Moreover, phages ϕEc1 and ϕEc3 decreased both biofilm formation and adherence, whereas ϕEc4 was able to decrease adherence but not biofilm formation. In conclusion, these novel phages showed the ability to decrease biofilm and bacterial adherence, making them promising candidates for effective adjuvant treatment against UTIs caused by MDR UPEC strains. Key points: Phage with lytic activity against MDR UPEC strains were isolated and characterized under in vitro conditions.A novel method was proposed to evaluate phage activity against bacterial adherence in HEp-2 cell.Phages represent a suitable strategy to control infections caused by MDR bacteria.
AB - Abstract: Urinary tract infections (UTIs) are mainly caused by uropathogenic Escherichia coli (UPEC), whose impact can be exacerbated by multidrug-resistant (MDR) strains. Effective control strategies are, therefore, urgently needed. Among them, phage therapy represents a suitable alternative. Here, we describe the isolation and characterization of novel phages from wastewater samples, as well as their lytic activity against biofilm and adherence of UPEC to HEp-2 cells. The results demonstrated that phage vB_EcoM-phiEc1 (ϕEc1) belongs to Myoviridae family, whereas vB_EcoS-phiEc3 (ϕEc3) and vB_EcoS-phiEc4 (ϕEc4) belong to Siphoviridae family. Phages showed lytic activity against UPEC and gut commensal strains. Phage ϕEc1 lysed UPEC serogroups, whereas phages ϕEc3 and ϕEc4 lysed only UTI strains with higher prevalence toward the O25 serogroup. Moreover, phages ϕEc1 and ϕEc3 decreased both biofilm formation and adherence, whereas ϕEc4 was able to decrease adherence but not biofilm formation. In conclusion, these novel phages showed the ability to decrease biofilm and bacterial adherence, making them promising candidates for effective adjuvant treatment against UTIs caused by MDR UPEC strains. Key points: Phage with lytic activity against MDR UPEC strains were isolated and characterized under in vitro conditions.A novel method was proposed to evaluate phage activity against bacterial adherence in HEp-2 cell.Phages represent a suitable strategy to control infections caused by MDR bacteria.
KW - Adherence
KW - Biofilm
KW - Multidrug resistance
KW - Phage therapy
KW - Urinary tract infections
UR - http://www.scopus.com/inward/record.url?scp=85110866066&partnerID=8YFLogxK
U2 - 10.1007/s00253-021-11432-6
DO - 10.1007/s00253-021-11432-6
M3 - Artículo
C2 - 34254156
AN - SCOPUS:85110866066
SN - 0175-7598
VL - 105
SP - 5617
EP - 5629
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
IS - 13
ER -