TY - JOUR
T1 - Isolation and characterization of functional insertion sequences of rhizobia
AU - Hernandez-Lucas, Ismael
AU - Ramirez-Trujillo, Jose Augusto
AU - Gaitan, Miguel Angel
AU - Guo, Xianwu
AU - Flores, Margarita
AU - Martinez-Romero, Esperanza
AU - Perez-Rueda, Ernesto
AU - Mavingui, Patrick
PY - 2006/8
Y1 - 2006/8
N2 - Rhizobia are a group of bacteria that form nodules on the roots of legume host plants. The sequenced genomes of the rhizobia are characterized by the presence of many putative insertion sequences (IS) elements. However, it is unknown whether these IS elements are functional and it is therefore relevant to assess their transposition activity. In this work, several functional insertion sequences belonging to the IS1256, IS3, IS5, IS166, and IS21 families were captured from Rhizobium tropici, Rhizobium sp. NGR234 and Sinorhizobium meliloti, using pGBG1 as a trapping system. In silico analysis shows that homologs of rhizobia mobile elements are present in distantly related genomes, suggesting that Rhizobium IS elements are prone to genetic transfer.
AB - Rhizobia are a group of bacteria that form nodules on the roots of legume host plants. The sequenced genomes of the rhizobia are characterized by the presence of many putative insertion sequences (IS) elements. However, it is unknown whether these IS elements are functional and it is therefore relevant to assess their transposition activity. In this work, several functional insertion sequences belonging to the IS1256, IS3, IS5, IS166, and IS21 families were captured from Rhizobium tropici, Rhizobium sp. NGR234 and Sinorhizobium meliloti, using pGBG1 as a trapping system. In silico analysis shows that homologs of rhizobia mobile elements are present in distantly related genomes, suggesting that Rhizobium IS elements are prone to genetic transfer.
KW - Insertion sequences
KW - Rhizobia
KW - Transposition
UR - http://www.scopus.com/inward/record.url?scp=33745684181&partnerID=8YFLogxK
U2 - 10.1111/j.1574-6968.2006.00319.x
DO - 10.1111/j.1574-6968.2006.00319.x
M3 - Artículo
C2 - 16842354
AN - SCOPUS:33745684181
SN - 0378-1097
VL - 261
SP - 25
EP - 31
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 1
ER -