TY - JOUR
T1 - Isolation and analysis of a new developmentally regulated gene from amastigotes of Leishmania mexicana mexicana
AU - Liu, Keyi
AU - Zinker, Samuel
AU - Argüello, Carlos
AU - Salgado, Luis Miguel
N1 - Funding Information:
Acknowledgements We would like to thank Dr. S.M. Landfear and Dr. M.A. Sanchez (Oregon Health Science University) for the kind gift of the L. mexicana-amastigote cDNA library and to express our appreciation to J.Z. Yu, A.J. Calderón, G. Aguilar, and V.J.N. Cirnes for their technical assistance. This project was partially supported by grant 98034R6/Bio/LA from TWAS and by grants 4386M and 26289N from CONACYT, México. Keyi Liu is the recipient of a scholarship from the Departamento de Genética y Biologṍ a Molecular, Centro de Investigación y de Estudios Avanzados del I.P.N., México. The gene sequence reported in this paper has been deposited in the GenBank data base (accession number AF032464).
PY - 2000
Y1 - 2000
N2 - Leishmania differentiates from the promastigote to the amastigote stage during its digenetic life cycle. Characterization of the developmentally regulated genes during that process would help to elucidate the mechanisms of gene regulation. In this study, specific fragments of mRNAs from the amastigote stage of L. mexicana mexicana were discriminated from those of the promastigote and metacyclic stages by differential display. This technique combined with spliced-leader polymerase chain reaction allowed isolation of the complete gene VG7A5. The sequence of this gene did not align with any published L. mexicana sequence. More than one copy of this gene was identified in the genome by Southern-blot analysis and was transcribed exclusively in the amastigote stage. At 20 bp upstream from the splice AG site it has a trans-splicing polypyrimidine tract. The gene encodes the subcellular localization motifs 5'-GGACT and AAGCT-3' in the 3' untranslated region of the mRNA. The open reading frame of the gene VG7A5 predicts a polypeptide of 587 amino acid residues that has a KGRR amidation motif near its carboxyl terminus, suggesting that in the mammalian host this protein may be involved in the process of acute inflammation.
AB - Leishmania differentiates from the promastigote to the amastigote stage during its digenetic life cycle. Characterization of the developmentally regulated genes during that process would help to elucidate the mechanisms of gene regulation. In this study, specific fragments of mRNAs from the amastigote stage of L. mexicana mexicana were discriminated from those of the promastigote and metacyclic stages by differential display. This technique combined with spliced-leader polymerase chain reaction allowed isolation of the complete gene VG7A5. The sequence of this gene did not align with any published L. mexicana sequence. More than one copy of this gene was identified in the genome by Southern-blot analysis and was transcribed exclusively in the amastigote stage. At 20 bp upstream from the splice AG site it has a trans-splicing polypyrimidine tract. The gene encodes the subcellular localization motifs 5'-GGACT and AAGCT-3' in the 3' untranslated region of the mRNA. The open reading frame of the gene VG7A5 predicts a polypeptide of 587 amino acid residues that has a KGRR amidation motif near its carboxyl terminus, suggesting that in the mammalian host this protein may be involved in the process of acute inflammation.
UR - http://www.scopus.com/inward/record.url?scp=0033813704&partnerID=8YFLogxK
U2 - 10.1007/s004360050023
DO - 10.1007/s004360050023
M3 - Artículo
SN - 0932-0113
VL - 86
SP - 140
EP - 150
JO - Parasitology Research
JF - Parasitology Research
IS - 2
ER -