TY - JOUR
T1 - Interaction of secretory immunoglobulin A antibodies with Naegleria fowleri trophozoites and collagen type I
AU - Shibayama, Mineko
AU - Serrano-Luna, José De Jesús
AU - Rojas-Hernández, Saúl
AU - Campos-Rodríguez, Rafael
AU - Tsutsumi, Víctor
PY - 2003/3/1
Y1 - 2003/3/1
N2 - In this work, we analyzed the in vitro interaction of human secretory immunoglobulin A (sIgA) antibodies with Naegleria fowleri trophozoites and the capacity of these antibodies to inhibit amoeba adherence to collagen type I. We also studied N. fowleri antigens that are recognized by sIgA, using immunoblot assays. Immunocytochemical analysis of the interaction showed a redistribution of antigens on the surface of trophozoites by sIgA antibodies. Ultrastructural analysis of antibody-amoeba interaction showed that besides the patching and cap formation, parasites were capable of eliminating the antigen-antibody complex produced on the surface. sIgA antibodies were capable of inhibiting the in vitro adhesion of trophozoites to collagen type I. We suggest that nonsymptomatic infections by N. fowleri may stimulate a local specific immunity that prevents trophozoite adhesion and invasion of nasal mucosa.
AB - In this work, we analyzed the in vitro interaction of human secretory immunoglobulin A (sIgA) antibodies with Naegleria fowleri trophozoites and the capacity of these antibodies to inhibit amoeba adherence to collagen type I. We also studied N. fowleri antigens that are recognized by sIgA, using immunoblot assays. Immunocytochemical analysis of the interaction showed a redistribution of antigens on the surface of trophozoites by sIgA antibodies. Ultrastructural analysis of antibody-amoeba interaction showed that besides the patching and cap formation, parasites were capable of eliminating the antigen-antibody complex produced on the surface. sIgA antibodies were capable of inhibiting the in vitro adhesion of trophozoites to collagen type I. We suggest that nonsymptomatic infections by N. fowleri may stimulate a local specific immunity that prevents trophozoite adhesion and invasion of nasal mucosa.
KW - Free-living amoebae
KW - Human antibodies
KW - Mucosal immunity
KW - Naegleria fowleri
KW - Secretory IgA
UR - http://www.scopus.com/inward/record.url?scp=0037872084&partnerID=8YFLogxK
U2 - 10.1139/w03-023
DO - 10.1139/w03-023
M3 - Artículo
SN - 0008-4166
VL - 49
SP - 164
EP - 170
JO - Canadian Journal of Microbiology
JF - Canadian Journal of Microbiology
IS - 3
ER -