Interaction of secretory immunoglobulin A antibodies with Naegleria fowleri trophozoites and collagen type I

Mineko Shibayama, José De Jesús Serrano-Luna, Saúl Rojas-Hernández, Rafael Campos-Rodríguez, Víctor Tsutsumi

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

In this work, we analyzed the in vitro interaction of human secretory immunoglobulin A (sIgA) antibodies with Naegleria fowleri trophozoites and the capacity of these antibodies to inhibit amoeba adherence to collagen type I. We also studied N. fowleri antigens that are recognized by sIgA, using immunoblot assays. Immunocytochemical analysis of the interaction showed a redistribution of antigens on the surface of trophozoites by sIgA antibodies. Ultrastructural analysis of antibody-amoeba interaction showed that besides the patching and cap formation, parasites were capable of eliminating the antigen-antibody complex produced on the surface. sIgA antibodies were capable of inhibiting the in vitro adhesion of trophozoites to collagen type I. We suggest that nonsymptomatic infections by N. fowleri may stimulate a local specific immunity that prevents trophozoite adhesion and invasion of nasal mucosa.
Original languageAmerican English
Pages (from-to)164-170
Number of pages146
JournalCanadian Journal of Microbiology
DOIs
StatePublished - 1 Mar 2003

Fingerprint

Naegleria fowleri
Secretory Immunoglobulin A
Trophozoites
Collagen Type I
Antibodies
Amoeba
Nasal Mucosa
Surface Antigens
Antigen-Antibody Complex
Immunity
Parasites
Antigens
Infection

Cite this

@article{e436d686ac8749638159879a4e944618,
title = "Interaction of secretory immunoglobulin A antibodies with Naegleria fowleri trophozoites and collagen type I",
abstract = "In this work, we analyzed the in vitro interaction of human secretory immunoglobulin A (sIgA) antibodies with Naegleria fowleri trophozoites and the capacity of these antibodies to inhibit amoeba adherence to collagen type I. We also studied N. fowleri antigens that are recognized by sIgA, using immunoblot assays. Immunocytochemical analysis of the interaction showed a redistribution of antigens on the surface of trophozoites by sIgA antibodies. Ultrastructural analysis of antibody-amoeba interaction showed that besides the patching and cap formation, parasites were capable of eliminating the antigen-antibody complex produced on the surface. sIgA antibodies were capable of inhibiting the in vitro adhesion of trophozoites to collagen type I. We suggest that nonsymptomatic infections by N. fowleri may stimulate a local specific immunity that prevents trophozoite adhesion and invasion of nasal mucosa.",
author = "Mineko Shibayama and Serrano-Luna, {Jos{\'e} De Jes{\'u}s} and Sa{\'u}l Rojas-Hern{\'a}ndez and Rafael Campos-Rodr{\'i}guez and V{\'i}ctor Tsutsumi",
year = "2003",
month = "3",
day = "1",
doi = "10.1139/w03-023",
language = "American English",
pages = "164--170",
journal = "Canadian Journal of Microbiology",
issn = "0008-4166",
publisher = "National Research Council of Canada",

}

Interaction of secretory immunoglobulin A antibodies with Naegleria fowleri trophozoites and collagen type I. / Shibayama, Mineko; Serrano-Luna, José De Jesús; Rojas-Hernández, Saúl; Campos-Rodríguez, Rafael; Tsutsumi, Víctor.

In: Canadian Journal of Microbiology, 01.03.2003, p. 164-170.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Interaction of secretory immunoglobulin A antibodies with Naegleria fowleri trophozoites and collagen type I

AU - Shibayama, Mineko

AU - Serrano-Luna, José De Jesús

AU - Rojas-Hernández, Saúl

AU - Campos-Rodríguez, Rafael

AU - Tsutsumi, Víctor

PY - 2003/3/1

Y1 - 2003/3/1

N2 - In this work, we analyzed the in vitro interaction of human secretory immunoglobulin A (sIgA) antibodies with Naegleria fowleri trophozoites and the capacity of these antibodies to inhibit amoeba adherence to collagen type I. We also studied N. fowleri antigens that are recognized by sIgA, using immunoblot assays. Immunocytochemical analysis of the interaction showed a redistribution of antigens on the surface of trophozoites by sIgA antibodies. Ultrastructural analysis of antibody-amoeba interaction showed that besides the patching and cap formation, parasites were capable of eliminating the antigen-antibody complex produced on the surface. sIgA antibodies were capable of inhibiting the in vitro adhesion of trophozoites to collagen type I. We suggest that nonsymptomatic infections by N. fowleri may stimulate a local specific immunity that prevents trophozoite adhesion and invasion of nasal mucosa.

AB - In this work, we analyzed the in vitro interaction of human secretory immunoglobulin A (sIgA) antibodies with Naegleria fowleri trophozoites and the capacity of these antibodies to inhibit amoeba adherence to collagen type I. We also studied N. fowleri antigens that are recognized by sIgA, using immunoblot assays. Immunocytochemical analysis of the interaction showed a redistribution of antigens on the surface of trophozoites by sIgA antibodies. Ultrastructural analysis of antibody-amoeba interaction showed that besides the patching and cap formation, parasites were capable of eliminating the antigen-antibody complex produced on the surface. sIgA antibodies were capable of inhibiting the in vitro adhesion of trophozoites to collagen type I. We suggest that nonsymptomatic infections by N. fowleri may stimulate a local specific immunity that prevents trophozoite adhesion and invasion of nasal mucosa.

UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0037872084&origin=inward

UR - https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=0037872084&origin=inward

U2 - 10.1139/w03-023

DO - 10.1139/w03-023

M3 - Article

SP - 164

EP - 170

JO - Canadian Journal of Microbiology

JF - Canadian Journal of Microbiology

SN - 0008-4166

ER -