Initial characterization of the microenvironment that regulates connective tissue degradation in amniochorion during normal human labor

Guadalupe Estrada-Gutierrez, Veronica Zaga, Marco Antonio Gonzalez-Jimenez, Jorge Beltran-Montoya, Rolando Maida-Claros, Silvia Giono-Cerezo, Felipe Vadillo-Ortega

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Abstract

Extracellular matrix degradation in fetal membranes leading to its rupture is coupled to myometrial activity and cervical ripening during human normal labor. Mechanisms which modulate collagen degradation in amniochorion during labor have not been elucidated. Initial characterization of the effect of different blood compartments on connective tissue degradation in amniochorion during human labor was explored. Amniochorion explants were stimulated with plasma of maternal venous blood, umbilical cord blood or placental blood, obtained from women with pregnancies at term, with or without labor. MMP-2 and MMP-9 activities were quantified in conditioned media by gelatin-zymography as an index of connective tissue degradation. Collagen content was measured in tissue explants and collagen fibrils distribution was examined by electron microscopy. Placental plasma from term pregnancies, with or without labor, is enriched with soluble signals that enhance the in vitro MMP-9 production by amniochorion. Accompanying ultrastructural distortion of collagen fibers and demonstration of collagen degradation fragments confirmed induction of extracellular matrix degradation. Control experiments in which MMP-9 activity was blocked with TIMP-1 resulted in inhibition of all the above mentioned changes. These results suggest that placental intervillous space is a functional compartment in which mediators capable to induce collagen degradation in amniochorion are selectively expressed during human labor. © 2005 Elsevier B.V./International Society of Matrix Biology. All rights reserved.
Original languageAmerican English
Pages (from-to)306-312
Number of pages274
JournalMatrix Biology
DOIs
StatePublished - 1 Jun 2005

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Connective Tissue
Collagen
Matrix Metalloproteinases
Extracellular Matrix
Cervical Ripening
Extraembryonic Membranes
Pregnancy
Tissue Inhibitor of Metalloproteinase-1
Gelatin
Conditioned Culture Medium
Fetal Blood
Rupture
Electron Microscopy
Mothers

Cite this

Estrada-Gutierrez, Guadalupe ; Zaga, Veronica ; Gonzalez-Jimenez, Marco Antonio ; Beltran-Montoya, Jorge ; Maida-Claros, Rolando ; Giono-Cerezo, Silvia ; Vadillo-Ortega, Felipe. / Initial characterization of the microenvironment that regulates connective tissue degradation in amniochorion during normal human labor. In: Matrix Biology. 2005 ; pp. 306-312.
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abstract = "Extracellular matrix degradation in fetal membranes leading to its rupture is coupled to myometrial activity and cervical ripening during human normal labor. Mechanisms which modulate collagen degradation in amniochorion during labor have not been elucidated. Initial characterization of the effect of different blood compartments on connective tissue degradation in amniochorion during human labor was explored. Amniochorion explants were stimulated with plasma of maternal venous blood, umbilical cord blood or placental blood, obtained from women with pregnancies at term, with or without labor. MMP-2 and MMP-9 activities were quantified in conditioned media by gelatin-zymography as an index of connective tissue degradation. Collagen content was measured in tissue explants and collagen fibrils distribution was examined by electron microscopy. Placental plasma from term pregnancies, with or without labor, is enriched with soluble signals that enhance the in vitro MMP-9 production by amniochorion. Accompanying ultrastructural distortion of collagen fibers and demonstration of collagen degradation fragments confirmed induction of extracellular matrix degradation. Control experiments in which MMP-9 activity was blocked with TIMP-1 resulted in inhibition of all the above mentioned changes. These results suggest that placental intervillous space is a functional compartment in which mediators capable to induce collagen degradation in amniochorion are selectively expressed during human labor. {\circledC} 2005 Elsevier B.V./International Society of Matrix Biology. All rights reserved.",
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Initial characterization of the microenvironment that regulates connective tissue degradation in amniochorion during normal human labor. / Estrada-Gutierrez, Guadalupe; Zaga, Veronica; Gonzalez-Jimenez, Marco Antonio; Beltran-Montoya, Jorge; Maida-Claros, Rolando; Giono-Cerezo, Silvia; Vadillo-Ortega, Felipe.

In: Matrix Biology, 01.06.2005, p. 306-312.

Research output: Contribution to journalArticle

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