TY - JOUR
T1 - Inhibition of polyamine oxidase activity affects tumor development during the maize-Ustilago maydis interaction
AU - Jasso-Robles, Francisco Ignacio
AU - Jiménez-Bremont, Juan Francisco
AU - Becerra-Flora, Alicia
AU - Juárez-Montiel, Margarita
AU - Gonzalez, María Elisa
AU - Pieckenstain, Fernando Luis
AU - García de la Cruz, Ramón Fernando
AU - Rodríguez-Kessler, Margarita
N1 - Publisher Copyright:
© 2016 Elsevier Masson SAS.
PY - 2016/5/1
Y1 - 2016/5/1
N2 - Ustilago maydis is a biotrophic plant pathogenic fungus that leads to tumor development in the aerial tissues of its host, Zea mays. These tumors are the result of cell hypertrophy and hyperplasia, and are accompanied by the reprograming of primary and secondary metabolism of infected plants. Up to now, little is known regarding key plant actors and their role in tumor development during the interaction with U. maydis. Polyamines are small aliphatic amines that regulate plant growth, development and stress responses. In a previous study, we found substantial increases of polyamine levels in tumors. In the present work, we describe the maize polyamine oxidase (PAO) gene family, its contribution to hydrogen peroxide (H2O2) production and its possible role in tumor development induced by U. maydis. Histochemical analysis revealed that chlorotic lesions and maize tumors induced by U. maydis accumulate H2O2 to significant levels. Maize plants inoculated with U. maydis and treated with the PAO inhibitor 1,8-diaminooctane exhibit a notable reduction of H2O2 accumulation in infected tissues and a significant drop in PAO activity. This treatment also reduced disease symptoms in infected plants. Finally, among six maize PAO genes only the ZmPAO1, which encodes an extracellular enzyme, is up-regulated in tumors. Our data suggest that H2O2 produced through PA catabolism by ZmPAO1 plays an important role in tumor development during the maize-U. maydis interaction.
AB - Ustilago maydis is a biotrophic plant pathogenic fungus that leads to tumor development in the aerial tissues of its host, Zea mays. These tumors are the result of cell hypertrophy and hyperplasia, and are accompanied by the reprograming of primary and secondary metabolism of infected plants. Up to now, little is known regarding key plant actors and their role in tumor development during the interaction with U. maydis. Polyamines are small aliphatic amines that regulate plant growth, development and stress responses. In a previous study, we found substantial increases of polyamine levels in tumors. In the present work, we describe the maize polyamine oxidase (PAO) gene family, its contribution to hydrogen peroxide (H2O2) production and its possible role in tumor development induced by U. maydis. Histochemical analysis revealed that chlorotic lesions and maize tumors induced by U. maydis accumulate H2O2 to significant levels. Maize plants inoculated with U. maydis and treated with the PAO inhibitor 1,8-diaminooctane exhibit a notable reduction of H2O2 accumulation in infected tissues and a significant drop in PAO activity. This treatment also reduced disease symptoms in infected plants. Finally, among six maize PAO genes only the ZmPAO1, which encodes an extracellular enzyme, is up-regulated in tumors. Our data suggest that H2O2 produced through PA catabolism by ZmPAO1 plays an important role in tumor development during the maize-U. maydis interaction.
KW - 1,8-Diaminooctane
KW - Biotic stress
KW - Cell elongation
KW - Hydrogen peroxide
KW - Plant tumors
KW - Polyamine oxidase
KW - Ustilago maydis
UR - http://www.scopus.com/inward/record.url?scp=84960408765&partnerID=8YFLogxK
U2 - 10.1016/j.plaphy.2016.02.019
DO - 10.1016/j.plaphy.2016.02.019
M3 - Artículo
SN - 0981-9428
VL - 102
SP - 115
EP - 124
JO - Plant Physiology and Biochemistry
JF - Plant Physiology and Biochemistry
ER -