TY - JOUR
T1 - Inhibition of mycobacterium bovis BCG-induced tumor necrosis factor alpha secretion in human cells by transforming growth factor β
AU - Méndez-Samperio, Patricia
AU - Hernandez-Garay, Marisol
AU - Nuñez Vazquez, Angela
PY - 1998
Y1 - 1998
N2 - The effect of exogenous transforming growth factor β (TGF-β) on Mycobacterium bovis BCG-induced tumor necrosis factor alpha (TNF-α) production by human mononuclear cells was studied. It was found that TNF-α production by human cells stimulated with BCG was significantly inhibited by TGF-β. The specificity of the observed inhibition was demonstrated, since the addition of an anti-TGF-β neutralizing monoclonal antibody completely reversed the inhibitory effect. Furthermore, the suppressive effect of TGF-β on TNF-α secretion in this system was not due to a direct cytotoxic effect, since cell viability was comparable in the presence or absence of TGF-β. Interestingly, our results demonstrated comparative suppressive effects of TGF-β and interleukin-10 on BCG-induced TNF-α secretion. Together, the data demonstrate, for the first time, that TGF-β inhibits BCG-induced TNF-α secretion by human cells.
AB - The effect of exogenous transforming growth factor β (TGF-β) on Mycobacterium bovis BCG-induced tumor necrosis factor alpha (TNF-α) production by human mononuclear cells was studied. It was found that TNF-α production by human cells stimulated with BCG was significantly inhibited by TGF-β. The specificity of the observed inhibition was demonstrated, since the addition of an anti-TGF-β neutralizing monoclonal antibody completely reversed the inhibitory effect. Furthermore, the suppressive effect of TGF-β on TNF-α secretion in this system was not due to a direct cytotoxic effect, since cell viability was comparable in the presence or absence of TGF-β. Interestingly, our results demonstrated comparative suppressive effects of TGF-β and interleukin-10 on BCG-induced TNF-α secretion. Together, the data demonstrate, for the first time, that TGF-β inhibits BCG-induced TNF-α secretion by human cells.
UR - http://www.scopus.com/inward/record.url?scp=2642614519&partnerID=8YFLogxK
U2 - 10.1128/cdli.5.4.588-591.1998
DO - 10.1128/cdli.5.4.588-591.1998
M3 - Estudio breve
SN - 1071-412X
VL - 5
SP - 588
EP - 591
JO - Clinical and Diagnostic Laboratory Immunology
JF - Clinical and Diagnostic Laboratory Immunology
IS - 4
ER -