TY - JOUR
T1 - Inhibition of mitomycin c-induced sister chromatid exchanges by vitamin c in vivo
AU - Rivas Olmedo, G.
AU - Díaz Barriga Arceo, S.
AU - Madrigal Bujaidar, E.
PY - 1992/2
Y1 - 1992/2
N2 - The aim of this experiment was to test the modulation of genotoxicity produced by vitamin C (V-C) challenged against mitomycin C (MMC) in vivo, by analyzing the sister chromatid exchanges (SCE) and cell proliferation kinetics. We used the mouse bone marrow cytogenetic method, and tested three dosages of V-C (3, 5, and 7 glkg of body weight), along with the appropriate positive (2 mg MMC/kg body weight) and negative V-C controls. The results showed that V-C caused a strong inhibition of SCEs induced by MMC in the three dosages administered. The highest dose <7 glkg) caused an SCE inhibition of 70.02%, while the lower ones caused an inhibition of 54.61% and 52.30%, respectively. It was also clear that V-C per se does not increase the level of SCEs in mouse bone marrow cells. On the other hand, V-C induced a slight decrease in cell proliferation that was stronger when combined with MMC. Our data suggest that V-C effectively inhibit the SCE damage in vivo, but caution must be taken because of the observed cytotoxicity.
AB - The aim of this experiment was to test the modulation of genotoxicity produced by vitamin C (V-C) challenged against mitomycin C (MMC) in vivo, by analyzing the sister chromatid exchanges (SCE) and cell proliferation kinetics. We used the mouse bone marrow cytogenetic method, and tested three dosages of V-C (3, 5, and 7 glkg of body weight), along with the appropriate positive (2 mg MMC/kg body weight) and negative V-C controls. The results showed that V-C caused a strong inhibition of SCEs induced by MMC in the three dosages administered. The highest dose <7 glkg) caused an SCE inhibition of 70.02%, while the lower ones caused an inhibition of 54.61% and 52.30%, respectively. It was also clear that V-C per se does not increase the level of SCEs in mouse bone marrow cells. On the other hand, V-C induced a slight decrease in cell proliferation that was stronger when combined with MMC. Our data suggest that V-C effectively inhibit the SCE damage in vivo, but caution must be taken because of the observed cytotoxicity.
UR - http://www.scopus.com/inward/record.url?scp=0026542837&partnerID=8YFLogxK
U2 - 10.1080/15287399209531599
DO - 10.1080/15287399209531599
M3 - Artículo
SN - 0098-4108
VL - 35
SP - 107
EP - 113
JO - Journal of Toxicology and Environmental Health
JF - Journal of Toxicology and Environmental Health
IS - 2
ER -