Inhibition of acetylcholinesterase activity by hidroalcoholic extract and their fractions of Bouvardia ternifolia (Cav.) Shcltdl (Rubiaceae)

Maribel Herrera-Ruiz; Giovanni García-Morales; Alejandro Zamilpa; Manasés González-Cortazar; Jaime Tortoriello; Elsa Ventura-Zapata; Enrique Jiménez-Ferrer

Inhibition of acetylcholinesterase activity by hidroalcoholic extract and their fractions of Bouvardia ternifolia (Cav.) Shcltdl (Rubiaceae)

Translated title of the contribution: Inhibition of acetylcholinesterase activity by hidroalcoholic extract and their fractions of Bouvardia ternifolia (Cav.) Shcltdl (Rubiaceae)

Maribel Herrera-Ruiz, Giovanni García-Morales, Alejandro Zamilpa, Manasés González-Cortazar, Jaime Tortoriello, Elsa Ventura-Zapata, Enrique Jiménez-Ferrer

Centro de Desarrollo de Productos Bióticos (CEPROBI)

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The hydroalcoholic extract (BtHA) and its fractions of Bouvardia ternifolia were evaluated as inhibitors of the activity of the acetylcholinesterase enzyme utilizing the in vitro method (Ellman). BtHA inhibited the acetylcholinesterase enzyme competitively (IC₅₀ = 0.6 μg/ml); the ethyl acetate fraction (BtF-AcOEt) caused mixed-type inhibition (IC₅₀ = 0.96 μg/ml). A fraction insoluble on methanol (Bt-Faq-1) showed a mixed-type inhibition (CI₅₀ = 0.96 μg/ml). Finally, the methanol-soluble fraction (Bt-Faq-2), presented complex, mixed-type inhibition that corresponds to the C5 system (α = 0.740 and β = 0.842). Rutin, quercetin, kaempferol and ursolic acid were detected by HPLC and the concentration of these compounds was different in each fraction.

Translated title of the contribution	Inhibition of acetylcholinesterase activity by hidroalcoholic extract and their fractions of Bouvardia ternifolia (Cav.) Shcltdl (Rubiaceae)
Original language	English
Pages (from-to)	526-541
Number of pages	16
Journal	Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas
Volume	11
Issue number	6
State	Published - 2012

Keywords

Acetylcholinesterase
Bouvardia ternifolia
Kaempferol
Quercetin
Rutin
Ursolic acid

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Herrera-Ruiz, M., García-Morales, G., Zamilpa, A., González-Cortazar, M., Tortoriello, J., Ventura-Zapata, E., & Jiménez-Ferrer, E. (2012). Inhibition of acetylcholinesterase activity by hidroalcoholic extract and their fractions of Bouvardia ternifolia (Cav.) Shcltdl (Rubiaceae). Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas, 11(6), 526-541.

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title = "Inhibition of acetylcholinesterase activity by hidroalcoholic extract and their fractions of Bouvardia ternifolia (Cav.) Shcltdl (Rubiaceae)",

abstract = "The hydroalcoholic extract (BtHA) and its fractions of Bouvardia ternifolia were evaluated as inhibitors of the activity of the acetylcholinesterase enzyme utilizing the in vitro method (Ellman). BtHA inhibited the acetylcholinesterase enzyme competitively (IC50 = 0.6 μg/ml); the ethyl acetate fraction (BtF-AcOEt) caused mixed-type inhibition (IC50 = 0.96 μg/ml). A fraction insoluble on methanol (Bt-Faq-1) showed a mixed-type inhibition (CI50 = 0.96 μg/ml). Finally, the methanol-soluble fraction (Bt-Faq-2), presented complex, mixed-type inhibition that corresponds to the C5 system (α = 0.740 and β = 0.842). Rutin, quercetin, kaempferol and ursolic acid were detected by HPLC and the concentration of these compounds was different in each fraction.",

keywords = "Acetylcholinesterase, Bouvardia ternifolia, Kaempferol, Quercetin, Rutin, Ursolic acid",

author = "Maribel Herrera-Ruiz and Giovanni Garc{\'i}a-Morales and Alejandro Zamilpa and Manas{\'e}s Gonz{\'a}lez-Cortazar and Jaime Tortoriello and Elsa Ventura-Zapata and Enrique Jim{\'e}nez-Ferrer",

year = "2012",

language = "Ingl{\'e}s",

volume = "11",

pages = "526--541",

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Herrera-Ruiz, M, García-Morales, G, Zamilpa, A, González-Cortazar, M, Tortoriello, J, Ventura-Zapata, E & Jiménez-Ferrer, E 2012, 'Inhibition of acetylcholinesterase activity by hidroalcoholic extract and their fractions of Bouvardia ternifolia (Cav.) Shcltdl (Rubiaceae)', Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas, vol. 11, no. 6, pp. 526-541.

Inhibition of acetylcholinesterase activity by hidroalcoholic extract and their fractions of Bouvardia ternifolia (Cav.) Shcltdl (Rubiaceae). / Herrera-Ruiz, Maribel; García-Morales, Giovanni; Zamilpa, Alejandro et al.
In: Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas, Vol. 11, No. 6, 2012, p. 526-541.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Inhibition of acetylcholinesterase activity by hidroalcoholic extract and their fractions of Bouvardia ternifolia (Cav.) Shcltdl (Rubiaceae)

AU - Herrera-Ruiz, Maribel

AU - García-Morales, Giovanni

AU - Zamilpa, Alejandro

AU - González-Cortazar, Manasés

AU - Tortoriello, Jaime

AU - Ventura-Zapata, Elsa

AU - Jiménez-Ferrer, Enrique

PY - 2012

Y1 - 2012

N2 - The hydroalcoholic extract (BtHA) and its fractions of Bouvardia ternifolia were evaluated as inhibitors of the activity of the acetylcholinesterase enzyme utilizing the in vitro method (Ellman). BtHA inhibited the acetylcholinesterase enzyme competitively (IC50 = 0.6 μg/ml); the ethyl acetate fraction (BtF-AcOEt) caused mixed-type inhibition (IC50 = 0.96 μg/ml). A fraction insoluble on methanol (Bt-Faq-1) showed a mixed-type inhibition (CI50 = 0.96 μg/ml). Finally, the methanol-soluble fraction (Bt-Faq-2), presented complex, mixed-type inhibition that corresponds to the C5 system (α = 0.740 and β = 0.842). Rutin, quercetin, kaempferol and ursolic acid were detected by HPLC and the concentration of these compounds was different in each fraction.

AB - The hydroalcoholic extract (BtHA) and its fractions of Bouvardia ternifolia were evaluated as inhibitors of the activity of the acetylcholinesterase enzyme utilizing the in vitro method (Ellman). BtHA inhibited the acetylcholinesterase enzyme competitively (IC50 = 0.6 μg/ml); the ethyl acetate fraction (BtF-AcOEt) caused mixed-type inhibition (IC50 = 0.96 μg/ml). A fraction insoluble on methanol (Bt-Faq-1) showed a mixed-type inhibition (CI50 = 0.96 μg/ml). Finally, the methanol-soluble fraction (Bt-Faq-2), presented complex, mixed-type inhibition that corresponds to the C5 system (α = 0.740 and β = 0.842). Rutin, quercetin, kaempferol and ursolic acid were detected by HPLC and the concentration of these compounds was different in each fraction.

KW - Acetylcholinesterase

KW - Bouvardia ternifolia

KW - Kaempferol

KW - Quercetin

KW - Rutin

KW - Ursolic acid

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M3 - Artículo

SN - 0717-7917

VL - 11

SP - 526

EP - 541

JO - Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas

JF - Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas

IS - 6

ER -

Inhibition of acetylcholinesterase activity by hidroalcoholic extract and their fractions of Bouvardia ternifolia (Cav.) Shcltdl (Rubiaceae)

Abstract

Keywords

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