TY - JOUR
T1 - Influence of levamisole and Freund's adjuvant on mouse immunisation with antigens of adults of the liver fluke Fasciola hepatica Linnaeus, 1758
AU - Gutiérrez-Sánchez, María De Los Ángeles
AU - Luna-Herrera, Julieta
AU - Trejo-Castro, Lauro
AU - Montenegro-Cristino, Natividad
AU - Almanza-González, Alfredo
AU - Escobar-Gutiérrez, Alejandro
AU - De La Rosa-Arana, Jorge Luis
N1 - Publisher Copyright:
© Institute of Parasitology, Biology Centre CAS.
PY - 2015
Y1 - 2015
N2 - We have studied the influence of both levamisole (AL) and Freund's adjuvant (AF) on the immunisation of mice with the secretory antigens of adults of the liver fluke Fasciola hepatica Linnaeus, 1758. Total IgG antibodies were detected in all groups where the F. hepatica antigen was administered, been levels of IgG1 increased respect to IgG2a antibodies. During immunisation, IL-4 and IFN-? were only detected in AL and AF groups, but after infection, IL-4 boosted in all groups. IFN-? increased two fold in AF and AL groups compared to the saline solution (AS) group. Worm recovering was of 32-35% in groups administered without antigen whereas in AS, AL and AF groups recovering was of 25%, 12% and 8%, respectively. Macroscopical lesions in the liver were scarce in AL and AF groups. Our data suggest that immunisation of mice with antigens of F. hepatica enhances the immune response avoiding both liver damage and worm establishment after challenge infection. The murine model of fasciolosis has appeared to be useful to elucidate the mechanism by which the parasite modulates immune responses toward a Th2 type but also the development of Th1 type-inducing vaccines.
AB - We have studied the influence of both levamisole (AL) and Freund's adjuvant (AF) on the immunisation of mice with the secretory antigens of adults of the liver fluke Fasciola hepatica Linnaeus, 1758. Total IgG antibodies were detected in all groups where the F. hepatica antigen was administered, been levels of IgG1 increased respect to IgG2a antibodies. During immunisation, IL-4 and IFN-? were only detected in AL and AF groups, but after infection, IL-4 boosted in all groups. IFN-? increased two fold in AF and AL groups compared to the saline solution (AS) group. Worm recovering was of 32-35% in groups administered without antigen whereas in AS, AL and AF groups recovering was of 25%, 12% and 8%, respectively. Macroscopical lesions in the liver were scarce in AL and AF groups. Our data suggest that immunisation of mice with antigens of F. hepatica enhances the immune response avoiding both liver damage and worm establishment after challenge infection. The murine model of fasciolosis has appeared to be useful to elucidate the mechanism by which the parasite modulates immune responses toward a Th2 type but also the development of Th1 type-inducing vaccines.
KW - Fasciolosis
KW - Helminth
KW - Immunity
KW - Immunomodulation
KW - Vaccine
UR - http://www.scopus.com/inward/record.url?scp=84982682337&partnerID=8YFLogxK
U2 - 10.14411/fp.2015.047
DO - 10.14411/fp.2015.047
M3 - Artículo
C2 - 26384366
SN - 0015-5683
VL - 62
JO - Folia Parasitologica
JF - Folia Parasitologica
M1 - 47
ER -