TY - JOUR
T1 - INDUCCION DE LA RESPUESTA INMUNE HUMORAL CONTRA SALMONELLA TYPHIMURIUM EN PLACAS DE PEYER DE RATON
AU - Villarreal, N. G.C.
AU - Ventura-Juarez, J.
AU - Castaneda-Ibarra, F. J.
AU - Ruiz-Maya, L.
AU - Garcia-Latorre, E.
AU - Campos-Rodriguez, R.
PY - 1995
Y1 - 1995
N2 - The mechanisms of immune response generation and regulation at the intestinal level are not well known, mainly due to the lack of suitable and reproducible methods to measure local immune responses. The Cunningham direct and indirect hemolytic plaque assay for the quantification of antibody producing cells against Salmonella in Peyer's patches of mice orally infected with Salmonella typhimurium was used. After infection IgM and IgG producing cells were determined on days 6, 9, 12 and 19. Specific antibody producing cells appeared after bacterial invasion of Peyer's patches, nine days after infection. At this time, there were more antibody producing cells in the distal part of the intestine, which correlated with a higher infection of these Peyer's patches as detected by bacterial culture. After day nine, the number of plaque forming cells was similar in both parts of the intestine. The peak of response was on day 12 and diminished on day 19. The number of IgM and IgG producing cells was similar in all days analyzed. Histological analysis of Peyer's patches of infected mice showed inflammation with disorganization and tumefaction.
AB - The mechanisms of immune response generation and regulation at the intestinal level are not well known, mainly due to the lack of suitable and reproducible methods to measure local immune responses. The Cunningham direct and indirect hemolytic plaque assay for the quantification of antibody producing cells against Salmonella in Peyer's patches of mice orally infected with Salmonella typhimurium was used. After infection IgM and IgG producing cells were determined on days 6, 9, 12 and 19. Specific antibody producing cells appeared after bacterial invasion of Peyer's patches, nine days after infection. At this time, there were more antibody producing cells in the distal part of the intestine, which correlated with a higher infection of these Peyer's patches as detected by bacterial culture. After day nine, the number of plaque forming cells was similar in both parts of the intestine. The peak of response was on day 12 and diminished on day 19. The number of IgM and IgG producing cells was similar in all days analyzed. Histological analysis of Peyer's patches of infected mice showed inflammation with disorganization and tumefaction.
UR - http://www.scopus.com/inward/record.url?scp=0029073814&partnerID=8YFLogxK
M3 - Artículo
C2 - 8552875
AN - SCOPUS:0029073814
SN - 0187-4640
VL - 37
SP - 153
EP - 160
JO - Revista latinoamericana de microbiologia
JF - Revista latinoamericana de microbiologia
IS - 2
ER -