TY - JOUR
T1 - Inactivation of HSV-2 by ascorbate-Cu(II) and its protecting evaluation in CF-1 mice against encephalitis
AU - Betanzos-Cabrera, Gabriel
AU - Ramírez, Francisco J.
AU - Muñoz, José L.
AU - Barrón, Blanca L.
AU - Maldonado, Rogelio
N1 - Funding Information:
We want to thank Brent Harker from The University of Notre Dame and Mario Cancino from ENCB for their valuable advice and assistance on this paper. Financial support for this work was provided by CONACYT grant 3071-M, DEPI; IPN, grants 952176, 952699 and PROMEP.
PY - 2004/9/15
Y1 - 2004/9/15
N2 - Ascorbate is an important antioxidant. However, in the presence of transition metals such as Cu(II) or Fe(III), it also has pro-oxidant capabilities. The effect of ascorbate-Cu(II) in the in vitro infection of herpes simplex virus type 2 (HSV-2) and its protecting effect in a murine model was investigated. HSV-2 was treated with different concentrations of ascorbate in the presence of Cu(II). A group of CF-1 mice were treated with the inactivated virus and other treated with maintenance medium containing only ascorbate-Cu(II). Weeks later, mice were challenged intranasally with infectious viruses. HSV-2 was completely inactivated by 2 mM ascorbate plus 1 mM Cu(II). Ascorbate or Cu(II) alone did not inactivate the virus. Compared with the control group, 60% of the immunized animals did not show any sign of encephalitis and survived the herpes virus infection, while a 7% survival rate was observed in the control group (P=0.056). We concluded that the in vitro treatment of HSV-2 with ascorbate-Cu(II) is not only able to inactivate the virus, but also suggested that the viral particles induced a protective response against herpes encephalitis. This inactivation may provide an alternative method to develop new agents therapeutics.
AB - Ascorbate is an important antioxidant. However, in the presence of transition metals such as Cu(II) or Fe(III), it also has pro-oxidant capabilities. The effect of ascorbate-Cu(II) in the in vitro infection of herpes simplex virus type 2 (HSV-2) and its protecting effect in a murine model was investigated. HSV-2 was treated with different concentrations of ascorbate in the presence of Cu(II). A group of CF-1 mice were treated with the inactivated virus and other treated with maintenance medium containing only ascorbate-Cu(II). Weeks later, mice were challenged intranasally with infectious viruses. HSV-2 was completely inactivated by 2 mM ascorbate plus 1 mM Cu(II). Ascorbate or Cu(II) alone did not inactivate the virus. Compared with the control group, 60% of the immunized animals did not show any sign of encephalitis and survived the herpes virus infection, while a 7% survival rate was observed in the control group (P=0.056). We concluded that the in vitro treatment of HSV-2 with ascorbate-Cu(II) is not only able to inactivate the virus, but also suggested that the viral particles induced a protective response against herpes encephalitis. This inactivation may provide an alternative method to develop new agents therapeutics.
KW - Ascorbate-Cu(II)
KW - Encephalitis
KW - HSV
KW - In vitro
KW - Inactivation
KW - Protection
UR - http://www.scopus.com/inward/record.url?scp=3843074237&partnerID=8YFLogxK
U2 - 10.1016/j.jviromet.2004.05.003
DO - 10.1016/j.jviromet.2004.05.003
M3 - Artículo
SN - 0166-0934
VL - 120
SP - 161
EP - 165
JO - Journal of Virological Methods
JF - Journal of Virological Methods
IS - 2
ER -