In vivo assessment of liver fibrosis using diffuse reflectance and fluorescence spectroscopy: A proof of concept

Diego Fabila, José Manuel de la Rosa, Suren Stolik, Edgard Moreno, Karina Suárez-álvarez, Giuliana López-Navarrete, Carolina Guzmán, Jesús Aguirre-García, Christian Acevedo-García, David Kershenobich, Galileo Escobedo

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A novel application of diffuse reflectance and fluorescence spectroscopy in the assessment of liver fibrosis is here reported. To induce different stages of liver fibrosis, a sufficient number of male Wistar rats were differentially exposed to chronic administration with carbon tetrachloride. Then, diffuse reflectance and fluorescence spectra were in vivo measured from the liver surface of each animal by a minimal invasive laparoscopic procedure. The liver fibrosis degree was conventionally determined by means of histological examination using the Mason's Trichrome stain, accompanied by hepatic expression of α-sma, and evaluation of the ALT/AST serum levels. The liver from rats exhibiting higher grades of fibrosis showed a significant increase in diffuse reflectance and fluorescence intensity when compared with control animals. At 365. nm, the diffuse reflectance spectrum exhibited an increase of 4 and 3-fold in mild and advanced fibrotic rats, respectively, when compared to the control group. Similarly, the fluorescence emission at 493. nm was 2-fold higher in fibrotic animals than in controls. By using fluorescence intensity, discrimination algorithms indicated 73% sensitivity and 94% specificity for recognition of hepatic fibrosis, while for diffuse reflectance, these values increased up to 85% and 100%, respectively. Taking into consideration there is a special need for developing new diagnostic approaches focused on detecting different stages of liver fibrosis with minimal invasiveness, these results suggest that diffuse reflectance and fluorescence spectroscopy could be worthy of further exploration in patients with liver disease. © 2012 Elsevier B.V.
Original languageAmerican English
Pages (from-to)376-382
Number of pages337
JournalPhotodiagnosis and Photodynamic Therapy
DOIs
StatePublished - 1 Dec 2012

Fingerprint

Fluorescence Spectrometry
Liver Cirrhosis
Fluorescence
Liver
Fibrosis
Carbon Tetrachloride
Wistar Rats
Liver Diseases
Sensitivity and Specificity
Control Groups
Serum

Cite this

Fabila, Diego ; de la Rosa, José Manuel ; Stolik, Suren ; Moreno, Edgard ; Suárez-álvarez, Karina ; López-Navarrete, Giuliana ; Guzmán, Carolina ; Aguirre-García, Jesús ; Acevedo-García, Christian ; Kershenobich, David ; Escobedo, Galileo. / In vivo assessment of liver fibrosis using diffuse reflectance and fluorescence spectroscopy: A proof of concept. In: Photodiagnosis and Photodynamic Therapy. 2012 ; pp. 376-382.
@article{df0243617c0842f7ba9465bf6c109dcb,
title = "In vivo assessment of liver fibrosis using diffuse reflectance and fluorescence spectroscopy: A proof of concept",
abstract = "A novel application of diffuse reflectance and fluorescence spectroscopy in the assessment of liver fibrosis is here reported. To induce different stages of liver fibrosis, a sufficient number of male Wistar rats were differentially exposed to chronic administration with carbon tetrachloride. Then, diffuse reflectance and fluorescence spectra were in vivo measured from the liver surface of each animal by a minimal invasive laparoscopic procedure. The liver fibrosis degree was conventionally determined by means of histological examination using the Mason's Trichrome stain, accompanied by hepatic expression of α-sma, and evaluation of the ALT/AST serum levels. The liver from rats exhibiting higher grades of fibrosis showed a significant increase in diffuse reflectance and fluorescence intensity when compared with control animals. At 365. nm, the diffuse reflectance spectrum exhibited an increase of 4 and 3-fold in mild and advanced fibrotic rats, respectively, when compared to the control group. Similarly, the fluorescence emission at 493. nm was 2-fold higher in fibrotic animals than in controls. By using fluorescence intensity, discrimination algorithms indicated 73{\%} sensitivity and 94{\%} specificity for recognition of hepatic fibrosis, while for diffuse reflectance, these values increased up to 85{\%} and 100{\%}, respectively. Taking into consideration there is a special need for developing new diagnostic approaches focused on detecting different stages of liver fibrosis with minimal invasiveness, these results suggest that diffuse reflectance and fluorescence spectroscopy could be worthy of further exploration in patients with liver disease. {\circledC} 2012 Elsevier B.V.",
author = "Diego Fabila and {de la Rosa}, {Jos{\'e} Manuel} and Suren Stolik and Edgard Moreno and Karina Su{\'a}rez-{\'a}lvarez and Giuliana L{\'o}pez-Navarrete and Carolina Guzm{\'a}n and Jes{\'u}s Aguirre-Garc{\'i}a and Christian Acevedo-Garc{\'i}a and David Kershenobich and Galileo Escobedo",
year = "2012",
month = "12",
day = "1",
doi = "10.1016/j.pdpdt.2012.05.002",
language = "American English",
pages = "376--382",
journal = "Photodiagnosis and Photodynamic Therapy",
issn = "1572-1000",
publisher = "Elsevier",

}

Fabila, D, de la Rosa, JM, Stolik, S, Moreno, E, Suárez-álvarez, K, López-Navarrete, G, Guzmán, C, Aguirre-García, J, Acevedo-García, C, Kershenobich, D & Escobedo, G 2012, 'In vivo assessment of liver fibrosis using diffuse reflectance and fluorescence spectroscopy: A proof of concept', Photodiagnosis and Photodynamic Therapy, pp. 376-382. https://doi.org/10.1016/j.pdpdt.2012.05.002

In vivo assessment of liver fibrosis using diffuse reflectance and fluorescence spectroscopy: A proof of concept. / Fabila, Diego; de la Rosa, José Manuel; Stolik, Suren; Moreno, Edgard; Suárez-álvarez, Karina; López-Navarrete, Giuliana; Guzmán, Carolina; Aguirre-García, Jesús; Acevedo-García, Christian; Kershenobich, David; Escobedo, Galileo.

In: Photodiagnosis and Photodynamic Therapy, 01.12.2012, p. 376-382.

Research output: Contribution to journalArticle

TY - JOUR

T1 - In vivo assessment of liver fibrosis using diffuse reflectance and fluorescence spectroscopy: A proof of concept

AU - Fabila, Diego

AU - de la Rosa, José Manuel

AU - Stolik, Suren

AU - Moreno, Edgard

AU - Suárez-álvarez, Karina

AU - López-Navarrete, Giuliana

AU - Guzmán, Carolina

AU - Aguirre-García, Jesús

AU - Acevedo-García, Christian

AU - Kershenobich, David

AU - Escobedo, Galileo

PY - 2012/12/1

Y1 - 2012/12/1

N2 - A novel application of diffuse reflectance and fluorescence spectroscopy in the assessment of liver fibrosis is here reported. To induce different stages of liver fibrosis, a sufficient number of male Wistar rats were differentially exposed to chronic administration with carbon tetrachloride. Then, diffuse reflectance and fluorescence spectra were in vivo measured from the liver surface of each animal by a minimal invasive laparoscopic procedure. The liver fibrosis degree was conventionally determined by means of histological examination using the Mason's Trichrome stain, accompanied by hepatic expression of α-sma, and evaluation of the ALT/AST serum levels. The liver from rats exhibiting higher grades of fibrosis showed a significant increase in diffuse reflectance and fluorescence intensity when compared with control animals. At 365. nm, the diffuse reflectance spectrum exhibited an increase of 4 and 3-fold in mild and advanced fibrotic rats, respectively, when compared to the control group. Similarly, the fluorescence emission at 493. nm was 2-fold higher in fibrotic animals than in controls. By using fluorescence intensity, discrimination algorithms indicated 73% sensitivity and 94% specificity for recognition of hepatic fibrosis, while for diffuse reflectance, these values increased up to 85% and 100%, respectively. Taking into consideration there is a special need for developing new diagnostic approaches focused on detecting different stages of liver fibrosis with minimal invasiveness, these results suggest that diffuse reflectance and fluorescence spectroscopy could be worthy of further exploration in patients with liver disease. © 2012 Elsevier B.V.

AB - A novel application of diffuse reflectance and fluorescence spectroscopy in the assessment of liver fibrosis is here reported. To induce different stages of liver fibrosis, a sufficient number of male Wistar rats were differentially exposed to chronic administration with carbon tetrachloride. Then, diffuse reflectance and fluorescence spectra were in vivo measured from the liver surface of each animal by a minimal invasive laparoscopic procedure. The liver fibrosis degree was conventionally determined by means of histological examination using the Mason's Trichrome stain, accompanied by hepatic expression of α-sma, and evaluation of the ALT/AST serum levels. The liver from rats exhibiting higher grades of fibrosis showed a significant increase in diffuse reflectance and fluorescence intensity when compared with control animals. At 365. nm, the diffuse reflectance spectrum exhibited an increase of 4 and 3-fold in mild and advanced fibrotic rats, respectively, when compared to the control group. Similarly, the fluorescence emission at 493. nm was 2-fold higher in fibrotic animals than in controls. By using fluorescence intensity, discrimination algorithms indicated 73% sensitivity and 94% specificity for recognition of hepatic fibrosis, while for diffuse reflectance, these values increased up to 85% and 100%, respectively. Taking into consideration there is a special need for developing new diagnostic approaches focused on detecting different stages of liver fibrosis with minimal invasiveness, these results suggest that diffuse reflectance and fluorescence spectroscopy could be worthy of further exploration in patients with liver disease. © 2012 Elsevier B.V.

UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84870311538&origin=inward

UR - https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84870311538&origin=inward

U2 - 10.1016/j.pdpdt.2012.05.002

DO - 10.1016/j.pdpdt.2012.05.002

M3 - Article

SP - 376

EP - 382

JO - Photodiagnosis and Photodynamic Therapy

JF - Photodiagnosis and Photodynamic Therapy

SN - 1572-1000

ER -