TY - JOUR
T1 - In-vitro model for the ultrastructural study of the formation of thrombi in human platelets
AU - Cerecedo, Doris
AU - González, Sirenia
AU - Mondragón, Mónica
AU - Reyes, Elba
AU - Mondragón, Ricardo
PY - 2006/3
Y1 - 2006/3
N2 - Platelets are cell fragments with dynamic properties involved in clot formation after tissue damage. Platelet activation causes a change in shape, secretion of intracellular granules and aggregation with each other through the cytoskeleton components and biochemical changes. Platelet adhesion, considered as the major event in haemostasis, has been studied in several in-vitro and in-vivo models to evaluate the feasible thrombogenicity of some materials, the dynamics of specific receptors, as well as the effect of different buffers and inhibitors in this process. In spite of the numerous reports about platelet activation, to date there is no information available about the fine structure of the platelet-platelet and platelet-substrate interactions. In the present report we describe an in-vitro system that allows the visualization of these interactions: platelets are adhered to an inert substrate, and interactions with suspended platelets as a process to initiate the formation of thrombi was followed by ultramicrotomy and transmission electron microscopy.
AB - Platelets are cell fragments with dynamic properties involved in clot formation after tissue damage. Platelet activation causes a change in shape, secretion of intracellular granules and aggregation with each other through the cytoskeleton components and biochemical changes. Platelet adhesion, considered as the major event in haemostasis, has been studied in several in-vitro and in-vivo models to evaluate the feasible thrombogenicity of some materials, the dynamics of specific receptors, as well as the effect of different buffers and inhibitors in this process. In spite of the numerous reports about platelet activation, to date there is no information available about the fine structure of the platelet-platelet and platelet-substrate interactions. In the present report we describe an in-vitro system that allows the visualization of these interactions: platelets are adhered to an inert substrate, and interactions with suspended platelets as a process to initiate the formation of thrombi was followed by ultramicrotomy and transmission electron microscopy.
KW - Adhesion
KW - In-vitro model
KW - Platelets
KW - Thrombi
KW - Ultrastructure
UR - http://www.scopus.com/inward/record.url?scp=33646104648&partnerID=8YFLogxK
U2 - 10.1097/01.mbc.0000214712.96244.47
DO - 10.1097/01.mbc.0000214712.96244.47
M3 - Artículo
SN - 0957-5235
VL - 17
SP - 161
EP - 164
JO - Blood Coagulation and Fibrinolysis
JF - Blood Coagulation and Fibrinolysis
IS - 2
ER -