TY - JOUR
T1 - In vitro effect of sodium fluoride on malondialdehyde concentration and on superoxide dismutase, catalase, and glutathione peroxidase in human erythrocytes
AU - Gutiérrez-Salinas, José
AU - García-Ortíz, Liliana
AU - Morales González, José A.
AU - Hernández-Rodríguez, Sergio
AU - Ramírez-García, Sotero
AU - Núñez-Ramos, Norma R.
AU - Madrigal-Santillán, Eduardo
PY - 2013
Y1 - 2013
N2 - The aim of this paper was to describe the in vitro effect of sodium fluoride (NaF) on the specific activity of the major erythrocyte antioxidant enzymes, as well as on the membrane malondialdehyde concentration, as indicators of oxidative stress. For this purpose, human erythrocytes were incubated with NaF (0, 7, 28, 56, and 100 g/mL) or NaF (100 g/mL) + vitamin E (1, 2.5, 5 and 10 g/mL). The malondialdehyde (MDA) concentration on the surface of the erythrocytes was determined, as were the enzymatic activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GlPx). Our results demonstrated that erythrocytes incubated with increasing NaF concentrations had an increased MDA concentration, along with decreased activity of antioxidant enzymes. The presence of vitamin E partially reversed the toxic effects of NaF on erythrocytes. These findings suggest that NaF induces oxidative stress in erythrocytes in vitro, and this stress is partially reversed by the presence of vitamin E.
AB - The aim of this paper was to describe the in vitro effect of sodium fluoride (NaF) on the specific activity of the major erythrocyte antioxidant enzymes, as well as on the membrane malondialdehyde concentration, as indicators of oxidative stress. For this purpose, human erythrocytes were incubated with NaF (0, 7, 28, 56, and 100 g/mL) or NaF (100 g/mL) + vitamin E (1, 2.5, 5 and 10 g/mL). The malondialdehyde (MDA) concentration on the surface of the erythrocytes was determined, as were the enzymatic activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GlPx). Our results demonstrated that erythrocytes incubated with increasing NaF concentrations had an increased MDA concentration, along with decreased activity of antioxidant enzymes. The presence of vitamin E partially reversed the toxic effects of NaF on erythrocytes. These findings suggest that NaF induces oxidative stress in erythrocytes in vitro, and this stress is partially reversed by the presence of vitamin E.
UR - http://www.scopus.com/inward/record.url?scp=84885663762&partnerID=8YFLogxK
U2 - 10.1155/2013/864718
DO - 10.1155/2013/864718
M3 - Artículo
C2 - 24223512
SN - 2356-6140
VL - 2013
JO - Scientific World Journal
JF - Scientific World Journal
M1 - 864718
ER -