TY - JOUR
T1 - In vitro activity of bedaquiline against rapidly growing nontuberculous mycobacteria
AU - Aguilar-Ayala, Diana A.
AU - Cnockaert, Margo
AU - André, Emmanuel
AU - Andries, Koen
AU - Gonzalez-Y-merchand, Jorge A.
AU - Vandamme, Peter
AU - Palomino, Juan Carlos
AU - Martin, Anandi
N1 - Publisher Copyright:
© 2017 The Authors.
PY - 2017/8
Y1 - 2017/8
N2 - Bedaquiline (BDQ) has been proven to be effective in the treatment of multidrug-resistant tuberculosis. We hypothesized that BDQ could be a potential agent to treat nontuberculous mycobacterial (NTM) infection. The objective of this study was to evaluate the in vitro activity of BDQ against rapidly growing mycobacteria by assessing the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) against 18 NTM strains. For MIC determination we performed the resazurin microtitre assay broth dilution, and for the MBC the c.f.u. was determined. BDQ exhibited a strong inhibitory effect against most NTM tested; however, for some NTM strains the MBC was significantly higher than the MIC. A new finding is that Mycobacterium flavescens has a mutation in the gene atpE associated with natural resistance to BDQ. These preliminary promising results demonstrate that BDQ could be potentially useful for the treatment of NTM.
AB - Bedaquiline (BDQ) has been proven to be effective in the treatment of multidrug-resistant tuberculosis. We hypothesized that BDQ could be a potential agent to treat nontuberculous mycobacterial (NTM) infection. The objective of this study was to evaluate the in vitro activity of BDQ against rapidly growing mycobacteria by assessing the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) against 18 NTM strains. For MIC determination we performed the resazurin microtitre assay broth dilution, and for the MBC the c.f.u. was determined. BDQ exhibited a strong inhibitory effect against most NTM tested; however, for some NTM strains the MBC was significantly higher than the MIC. A new finding is that Mycobacterium flavescens has a mutation in the gene atpE associated with natural resistance to BDQ. These preliminary promising results demonstrate that BDQ could be potentially useful for the treatment of NTM.
KW - AtpE mutation
KW - Minimum bactericidal concentration
KW - Minimum inhibitory concentration
KW - Mycobacterium flavescens
KW - Nontuberculous mycobacteria
KW - Resistance to bedaquiline
UR - http://www.scopus.com/inward/record.url?scp=85028638295&partnerID=8YFLogxK
U2 - 10.1099/jmm.0.000537
DO - 10.1099/jmm.0.000537
M3 - Artículo
C2 - 28749330
SN - 0022-2615
VL - 66
SP - 1140
EP - 1143
JO - Journal of Medical Microbiology
JF - Journal of Medical Microbiology
IS - 8
ER -