TY - JOUR
T1 - In silico identification of highly conserved epitopes of influenza A H1N1, H2N2, H3N2, and H5N1 with diagnostic and vaccination potential
AU - Muñoz-Medina, José Esteban
AU - Sánchez-Vallejo, Carlos Javier
AU - Méndez-Tenorio, Alfonso
AU - Monroy-Muñoz, Irma Eloísa
AU - Angeles-Martínez, Javier
AU - Santos Coy-Arechavaleta, Andrea
AU - Santacruz-Tinoco, Clara Esperanza
AU - González-Ibarra, Joaquín
AU - Anguiano-Hernández, Yu Mei
AU - González-Bonilla, César Raúl
AU - Ramón-Gallegos, Eva
AU - Díaz-Quiñonez, José Alberto
N1 - Publisher Copyright:
© 2015 José Esteban Muñoz-Medina et al.
PY - 2015
Y1 - 2015
N2 - The unpredictable, evolutionary nature of the influenza A virus (IAV) is the primary problem when generating a vaccine and when designing diagnostic strategies; thus, it is necessary to determine the constant regions in viral proteins. In this study, we completed an in silico analysis of the reported epitopes of the 4 IAV proteins that are antigenically most significant (HA, NA, NP, and M2) in the 3 strains with the greatest world circulation in the last century (H1N1, H2N2, and H3N2) and in one of the main aviary subtypes responsible for zoonosis (H5N1). For this purpose, the HMMER program was used to align 3,016 epitopes reported in the Immune Epitope Database and Analysis Resource (IEDB) and distributed in 34,294 stored sequences in the Pfam database. Eighteen epitopes were identified: 8 in HA, 5 in NA, 3 in NP, and 2 in M2. These epitopes have remained constant since they were first identified (91 years) and are present in strains that have circulated on 5 continents. These sites could be targets for vaccination design strategies based on epitopes and/or as markers in the implementation of diagnostic techniques.
AB - The unpredictable, evolutionary nature of the influenza A virus (IAV) is the primary problem when generating a vaccine and when designing diagnostic strategies; thus, it is necessary to determine the constant regions in viral proteins. In this study, we completed an in silico analysis of the reported epitopes of the 4 IAV proteins that are antigenically most significant (HA, NA, NP, and M2) in the 3 strains with the greatest world circulation in the last century (H1N1, H2N2, and H3N2) and in one of the main aviary subtypes responsible for zoonosis (H5N1). For this purpose, the HMMER program was used to align 3,016 epitopes reported in the Immune Epitope Database and Analysis Resource (IEDB) and distributed in 34,294 stored sequences in the Pfam database. Eighteen epitopes were identified: 8 in HA, 5 in NA, 3 in NP, and 2 in M2. These epitopes have remained constant since they were first identified (91 years) and are present in strains that have circulated on 5 continents. These sites could be targets for vaccination design strategies based on epitopes and/or as markers in the implementation of diagnostic techniques.
UR - http://www.scopus.com/inward/record.url?scp=84939824943&partnerID=8YFLogxK
U2 - 10.1155/2015/813047
DO - 10.1155/2015/813047
M3 - Artículo
C2 - 26346523
AN - SCOPUS:84939824943
SN - 2314-6133
VL - 2015
JO - BioMed Research International
JF - BioMed Research International
M1 - 813047
ER -