Improving dengue virus diagnosis in rural areas of Mexico

M. M.B. Moreno-Altamirano; F. J. Sańchez-Garciá; I. Loṕez-Martińez; C. Rosales-Jimeńez; M. Vaźquez-Pichardo; L. J. Arriaga-Valona; F. Capitan-Ortega

Improving dengue virus diagnosis in rural areas of Mexico

M. M.B. Moreno-Altamirano, F. J. Sańchez-Garciá, I. Loṕez-Martińez, C. Rosales-Jimeńez, M. Vaźquez-Pichardo, L. J. Arriaga-Valona, F. Capitan-Ortega

Escuela Nacional de Ciencias Biológicas (ENCB)

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Evaluation of an "in-house system" for the diagnosis of dengue infection by detection of specific IgM and IgG antibodies showed that 25 out of 34 (73.53%) serum samples were positive for IgM antibodies; 6 (17.64%) were positive for IgG and 3 (8.8%) were negative for both IgM and IgG anti-DENV antibodies. Ten samples from "non-symptomatic" people were all negative. In order to evaluate the anti-DENV ELISA, 20 serum samples obtained from healthy individuals from a non-endemic region (Mexico City) and 20 serum samples previously classified as positive were tested. All 20 samples from healthy individuals proved to be negative for both IgM and IgG anti-DENV antibodies, whereas not all positive samples resulted as positive in our assay.

Original language	English
Pages (from-to)	87-94
Number of pages	8
Journal	Dengue Bulletin
Volume	33
Issue number	1
State	Published - Dec 2009

Keywords

Dengue
Endemic
In-house diagnosis kit
Mexico
Surveillance
Tamiahua

Cite this

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abstract = "Evaluation of an {"}in-house system{"} for the diagnosis of dengue infection by detection of specific IgM and IgG antibodies showed that 25 out of 34 (73.53%) serum samples were positive for IgM antibodies; 6 (17.64%) were positive for IgG and 3 (8.8%) were negative for both IgM and IgG anti-DENV antibodies. Ten samples from {"}non-symptomatic{"} people were all negative. In order to evaluate the anti-DENV ELISA, 20 serum samples obtained from healthy individuals from a non-endemic region (Mexico City) and 20 serum samples previously classified as positive were tested. All 20 samples from healthy individuals proved to be negative for both IgM and IgG anti-DENV antibodies, whereas not all positive samples resulted as positive in our assay.",

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T1 - Improving dengue virus diagnosis in rural areas of Mexico

AU - Moreno-Altamirano, M. M.B.

AU - Sańchez-Garciá, F. J.

AU - Loṕez-Martińez, I.

AU - Rosales-Jimeńez, C.

AU - Vaźquez-Pichardo, M.

AU - Arriaga-Valona, L. J.

AU - Capitan-Ortega, F.

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N2 - Evaluation of an "in-house system" for the diagnosis of dengue infection by detection of specific IgM and IgG antibodies showed that 25 out of 34 (73.53%) serum samples were positive for IgM antibodies; 6 (17.64%) were positive for IgG and 3 (8.8%) were negative for both IgM and IgG anti-DENV antibodies. Ten samples from "non-symptomatic" people were all negative. In order to evaluate the anti-DENV ELISA, 20 serum samples obtained from healthy individuals from a non-endemic region (Mexico City) and 20 serum samples previously classified as positive were tested. All 20 samples from healthy individuals proved to be negative for both IgM and IgG anti-DENV antibodies, whereas not all positive samples resulted as positive in our assay.

AB - Evaluation of an "in-house system" for the diagnosis of dengue infection by detection of specific IgM and IgG antibodies showed that 25 out of 34 (73.53%) serum samples were positive for IgM antibodies; 6 (17.64%) were positive for IgG and 3 (8.8%) were negative for both IgM and IgG anti-DENV antibodies. Ten samples from "non-symptomatic" people were all negative. In order to evaluate the anti-DENV ELISA, 20 serum samples obtained from healthy individuals from a non-endemic region (Mexico City) and 20 serum samples previously classified as positive were tested. All 20 samples from healthy individuals proved to be negative for both IgM and IgG anti-DENV antibodies, whereas not all positive samples resulted as positive in our assay.

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KW - Endemic

KW - In-house diagnosis kit

KW - Mexico

KW - Surveillance

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Improving dengue virus diagnosis in rural areas of Mexico

Abstract

Keywords

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