Improved extraction of bioactive compounds from biomass of the marine dinoflagellate microalga Amphidinium carterae

M. López-Rodríguez; M. C. Cerón-García; L. López-Rosales; E. Navarro-López; A. Sánchez-Mirón; A. Molina-Miras; A. C. Abreu; Ignacio Fernández; F. García-Camacho

doi:10.1016/j.biortech.2020.123518

Improved extraction of bioactive compounds from biomass of the marine dinoflagellate microalga Amphidinium carterae

M. López-Rodríguez, M. C. Cerón-García, L. López-Rosales, E. Navarro-López, A. Sánchez-Mirón, A. Molina-Miras, A. C. Abreu, Ignacio Fernández, F. García-Camacho

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

The extraction of three families of compounds (carotenoids, fatty acids and amphidinols) from the biomass of two strains of Amphidinium carterae (ACRN03 and Dn241EHU) was improved by tuning cell disruption and solvent extraction operations. The extraction of carotenoids was evaluated using alkaline saponification (0%-60% KOH d.w.) at different temperatures (25–80 °C). High levels of carotenoids were obtained at 60 °C using freeze-dried biomass, not subjected to cell disruption methods. The ACRN03 strain required 20% KOH whereas the Dn241EHU strain did not require saponification since carotenoid degradation was observed. The extraction efficiencies were determined with a wide range of pure solvents and mixtures thereof. Two empirical non-linear equations were used to correlate extraction percentages for each family of compounds with the Hildebrand solubility parameter (δ_T) and the polarity index of the solvents (PI). Thresholds of δ_T and PI of around 20 MPa^1/2 and 6, respectively, were determined for the extraction of amphidinols, consistent with antiproliferative activity measurements.

Original language	English
Article number	123518
Journal	Bioresource Technology
Volume	313
DOIs	https://doi.org/10.1016/j.biortech.2020.123518
State	Published - Oct 2020
Externally published	Yes

Keywords

Amphidinols
Carotenoids
Extraction process
Polyunsaturated fatty acids
Solubility parameters

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.biortech.2020.123518

Cite this

López-Rodríguez, M., Cerón-García, M. C., López-Rosales, L., Navarro-López, E., Sánchez-Mirón, A., Molina-Miras, A., Abreu, A. C., Fernández, I., & García-Camacho, F. (2020). Improved extraction of bioactive compounds from biomass of the marine dinoflagellate microalga Amphidinium carterae. Bioresource Technology, 313, Article 123518. https://doi.org/10.1016/j.biortech.2020.123518

@article{4e3ae24947064677bd40e675a1799972,

title = "Improved extraction of bioactive compounds from biomass of the marine dinoflagellate microalga Amphidinium carterae",

abstract = "The extraction of three families of compounds (carotenoids, fatty acids and amphidinols) from the biomass of two strains of Amphidinium carterae (ACRN03 and Dn241EHU) was improved by tuning cell disruption and solvent extraction operations. The extraction of carotenoids was evaluated using alkaline saponification (0%-60% KOH d.w.) at different temperatures (25–80 °C). High levels of carotenoids were obtained at 60 °C using freeze-dried biomass, not subjected to cell disruption methods. The ACRN03 strain required 20% KOH whereas the Dn241EHU strain did not require saponification since carotenoid degradation was observed. The extraction efficiencies were determined with a wide range of pure solvents and mixtures thereof. Two empirical non-linear equations were used to correlate extraction percentages for each family of compounds with the Hildebrand solubility parameter (δT) and the polarity index of the solvents (PI). Thresholds of δT and PI of around 20 MPa1/2 and 6, respectively, were determined for the extraction of amphidinols, consistent with antiproliferative activity measurements.",

keywords = "Amphidinols, Carotenoids, Extraction process, Polyunsaturated fatty acids, Solubility parameters",

author = "M. L{\'o}pez-Rodr{\'i}guez and Cer{\'o}n-Garc{\'i}a, {M. C.} and L. L{\'o}pez-Rosales and E. Navarro-L{\'o}pez and A. S{\'a}nchez-Mir{\'o}n and A. Molina-Miras and Abreu, {A. C.} and Ignacio Fern{\'a}ndez and F. Garc{\'i}a-Camacho",

note = "Publisher Copyright: {\textcopyright} 2020 Elsevier Ltd",

year = "2020",

month = oct,

doi = "10.1016/j.biortech.2020.123518",

language = "Ingl{\'e}s",

volume = "313",

journal = "Bioresource Technology",

issn = "0960-8524",

}

López-Rodríguez, M, Cerón-García, MC, López-Rosales, L, Navarro-López, E, Sánchez-Mirón, A, Molina-Miras, A, Abreu, AC, Fernández, I & García-Camacho, F 2020, 'Improved extraction of bioactive compounds from biomass of the marine dinoflagellate microalga Amphidinium carterae', Bioresource Technology, vol. 313, 123518. https://doi.org/10.1016/j.biortech.2020.123518

TY - JOUR

T1 - Improved extraction of bioactive compounds from biomass of the marine dinoflagellate microalga Amphidinium carterae

AU - López-Rodríguez, M.

AU - Cerón-García, M. C.

AU - López-Rosales, L.

AU - Navarro-López, E.

AU - Sánchez-Mirón, A.

AU - Molina-Miras, A.

AU - Abreu, A. C.

AU - Fernández, Ignacio

AU - García-Camacho, F.

PY - 2020/10

Y1 - 2020/10

N2 - The extraction of three families of compounds (carotenoids, fatty acids and amphidinols) from the biomass of two strains of Amphidinium carterae (ACRN03 and Dn241EHU) was improved by tuning cell disruption and solvent extraction operations. The extraction of carotenoids was evaluated using alkaline saponification (0%-60% KOH d.w.) at different temperatures (25–80 °C). High levels of carotenoids were obtained at 60 °C using freeze-dried biomass, not subjected to cell disruption methods. The ACRN03 strain required 20% KOH whereas the Dn241EHU strain did not require saponification since carotenoid degradation was observed. The extraction efficiencies were determined with a wide range of pure solvents and mixtures thereof. Two empirical non-linear equations were used to correlate extraction percentages for each family of compounds with the Hildebrand solubility parameter (δT) and the polarity index of the solvents (PI). Thresholds of δT and PI of around 20 MPa1/2 and 6, respectively, were determined for the extraction of amphidinols, consistent with antiproliferative activity measurements.

AB - The extraction of three families of compounds (carotenoids, fatty acids and amphidinols) from the biomass of two strains of Amphidinium carterae (ACRN03 and Dn241EHU) was improved by tuning cell disruption and solvent extraction operations. The extraction of carotenoids was evaluated using alkaline saponification (0%-60% KOH d.w.) at different temperatures (25–80 °C). High levels of carotenoids were obtained at 60 °C using freeze-dried biomass, not subjected to cell disruption methods. The ACRN03 strain required 20% KOH whereas the Dn241EHU strain did not require saponification since carotenoid degradation was observed. The extraction efficiencies were determined with a wide range of pure solvents and mixtures thereof. Two empirical non-linear equations were used to correlate extraction percentages for each family of compounds with the Hildebrand solubility parameter (δT) and the polarity index of the solvents (PI). Thresholds of δT and PI of around 20 MPa1/2 and 6, respectively, were determined for the extraction of amphidinols, consistent with antiproliferative activity measurements.

KW - Amphidinols

KW - Carotenoids

KW - Extraction process

KW - Polyunsaturated fatty acids

KW - Solubility parameters

UR - http://www.scopus.com/inward/record.url?scp=85085967648&partnerID=8YFLogxK

U2 - 10.1016/j.biortech.2020.123518

DO - 10.1016/j.biortech.2020.123518

M3 - Artículo

C2 - 32512427

AN - SCOPUS:85085967648

SN - 0960-8524

VL - 313

JO - Bioresource Technology

JF - Bioresource Technology

M1 - 123518

ER -

Improved extraction of bioactive compounds from biomass of the marine dinoflagellate microalga Amphidinium carterae

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this